Abstract：
Nowadays, proinflammatory factors are considered to play an important role in the pathophysiology of threatened preterm labor or chorioamnionitis. The aim of this study was to establish the normal reference range for interleukin-6 (IL-6) levels in the amniotic fluid and to identify factors which may alter this value.
Prospective study in a tertiary-level center including asymptomatic pregnant women undergoing amniocentesis for genetic studies from October 2016 to September 2019. IL-6 measurements in amniotic fluid were performed using a fluorescence immunoassay with microfluidic technology (ELLA Proteinsimple, Bio Techne). Maternal history and pregnancy data were also recorded.
This study included 140 pregnant women. Of those, women who underwent termination of pregnancy were excluded. Therefore, a total of 98 pregnancies were included in the final statistical analysis. The mean gestational age was 21.86 weeks (range: 15-38.7) at the time of amniocentesis, and 38.6 weeks (range: 30.9-41.4) at delivery. No cases of chorioamnionitis were reported. The log10 IL-6 values follow a normal distribution (W = 0.990, p = 0.692). The median, and the 5th, 10th, 90th, and 95th percentiles for IL-6 levels were 573, 105, 130, 1645, and 2260 pg/mL, respectively. The log10 IL-6 values were not affected by gestational age (p = 0.395), maternal age (p = 0.376), body mass index (p = 0.551), ethnicity (p = 0.467), smoking status (p = 0.933), parity (p = 0.557), method of conception (p = 0.322), or diabetes mellitus (p = 0.381).
The log10 IL-6 values follow a normal distribution. IL-6 values are independent of gestational age, maternal age, body mass index, ethnicity, smoking status, parity and method of conception. Our study provides a normal reference range for IL-6 levels in the amniotic fluid that can be used in future studies. We also observed that normal IL-6 values were higher in the amniotic fluid than in serum.
Prospective study in a tertiary-level center including asymptomatic pregnant women undergoing amniocentesis for genetic studies from October 2016 to September 2019. IL-6 measurements in amniotic fluid were performed using a fluorescence immunoassay with microfluidic technology (ELLA Proteinsimple, Bio Techne). Maternal history and pregnancy data were also recorded.
This study included 140 pregnant women. Of those, women who underwent termination of pregnancy were excluded. Therefore, a total of 98 pregnancies were included in the final statistical analysis. The mean gestational age was 21.86 weeks (range: 15-38.7) at the time of amniocentesis, and 38.6 weeks (range: 30.9-41.4) at delivery. No cases of chorioamnionitis were reported. The log10 IL-6 values follow a normal distribution (W = 0.990, p = 0.692). The median, and the 5th, 10th, 90th, and 95th percentiles for IL-6 levels were 573, 105, 130, 1645, and 2260 pg/mL, respectively. The log10 IL-6 values were not affected by gestational age (p = 0.395), maternal age (p = 0.376), body mass index (p = 0.551), ethnicity (p = 0.467), smoking status (p = 0.933), parity (p = 0.557), method of conception (p = 0.322), or diabetes mellitus (p = 0.381).
The log10 IL-6 values follow a normal distribution. IL-6 values are independent of gestational age, maternal age, body mass index, ethnicity, smoking status, parity and method of conception. Our study provides a normal reference range for IL-6 levels in the amniotic fluid that can be used in future studies. We also observed that normal IL-6 values were higher in the amniotic fluid than in serum.
摘要：
背景：如今，促炎因子被认为在先兆早产或绒毛膜羊膜炎的病理生理中起重要作用。这项研究的目的是建立羊水中白介素6（IL-6）水平的正常参考范围，并确定可能改变该值的因素。
方法：2016年10月至2019年9月在三级中心进行前瞻性研究，包括无症状孕妇进行羊膜穿刺术进行遗传研究。羊水中的IL-6测量是使用微流体技术的荧光免疫分析法（ELLAProteinsimple，生物技术公司）。还记录了产妇的病史和妊娠数据。
结果：这项研究包括140名孕妇。其中,终止妊娠的女性被排除在外.因此,共有98例妊娠纳入最终统计分析.羊膜穿刺术时的平均胎龄为21.86周（范围：15-38.7），交付时38.6周（范围：30.9-41.4）。无绒毛膜羊膜炎病例报告。log10IL-6值服从正态分布(W=0.990,p=0.692)。中位数，第五,第十,第90,IL-6水平的第95百分位数分别为573、105、130、1645和2260pg/mL，分别。log10IL-6值不受胎龄的影响（p=0.395），产妇年龄（p=0.376），体重指数（p=0.551），种族（p=0.467），吸烟状况（p=0.933），奇偶校验（p=0.557），概念方法(p=0.322)，或糖尿病（p=0.381）。
结论：log10IL-6值服从正态分布。IL-6值与胎龄无关，产妇年龄,身体质量指数,种族,吸烟状况,平价和概念方法。我们的研究提供了羊水中IL-6水平的正常参考范围，可用于未来的研究。我们还观察到羊水中的正常IL-6值高于血清。
方法：2016年10月至2019年9月在三级中心进行前瞻性研究，包括无症状孕妇进行羊膜穿刺术进行遗传研究。羊水中的IL-6测量是使用微流体技术的荧光免疫分析法（ELLAProteinsimple，生物技术公司）。还记录了产妇的病史和妊娠数据。
结果：这项研究包括140名孕妇。其中,终止妊娠的女性被排除在外.因此,共有98例妊娠纳入最终统计分析.羊膜穿刺术时的平均胎龄为21.86周（范围：15-38.7），交付时38.6周（范围：30.9-41.4）。无绒毛膜羊膜炎病例报告。log10IL-6值服从正态分布(W=0.990,p=0.692)。中位数，第五,第十,第90,IL-6水平的第95百分位数分别为573、105、130、1645和2260pg/mL，分别。log10IL-6值不受胎龄的影响（p=0.395），产妇年龄（p=0.376），体重指数（p=0.551），种族（p=0.467），吸烟状况（p=0.933），奇偶校验（p=0.557），概念方法(p=0.322)，或糖尿病（p=0.381）。
结论：log10IL-6值服从正态分布。IL-6值与胎龄无关，产妇年龄,身体质量指数,种族,吸烟状况,平价和概念方法。我们的研究提供了羊水中IL-6水平的正常参考范围，可用于未来的研究。我们还观察到羊水中的正常IL-6值高于血清。
