PDF(Pubmed)
Abstract：
UNASSIGNED: During plant evolution, nucleotide-binding sites (NBS) and leucine-rich repeat (LRR) genes have made significant contributions to plant disease resistance. With many high-quality plant genomes sequenced, identification and comprehensive analyses of NBS-LRR genes at whole genome level are of great importance to understand and utilize them.
UNASSIGNED: In this study, we identified the NBS-LRR genes of 23 representative species at whole genome level, and researches on NBS-LRR genes of four monocotyledonous grass species, Saccharum spontaneum, Saccharum officinarum, Sorghum bicolor and Miscanthus sinensis, were focused.
UNASSIGNED: We found that whole genome duplication, gene expansion, and allele loss could be factors affecting the number of NBS-LRR genes in the species, and whole genome duplication is likely to be the main cause of the number of NBS-LRR genes in sugarcane. Meanwhile, we also found a progressive trend of positive selection on NBS-LRR genes. These studies further elucidated the evolutionary pattern of NBS-LRR genes in plants. Transcriptome data from multiple sugarcane diseases revealed that more differentially expressed NBS-LRR genes were derived from S. spontaneum than from S. officinarum in modern sugarcane cultivars, and the proportion was significantly higher than the expected. This finding reveals that S. spontaneum has a greater contribution to disease resistance for modern sugarcane cultivars. In addition, we observed allelespecific expression of seven NBS-LRR genes under leaf scald, and 125 NBS-LRR genes responding to multiple diseases were identified. Finally, we built a plant NBS-LRR gene database to facilitate subsequent analysis and use of NBSLRR genes obtained here. In conclusion, this study complemented and completed the research of plant NBS-LRR genes, and discussed how NBS-LRR genes responding to sugarcane diseases, which provided a guide and genetic resources for further research and utilization of NBS-LRR genes.
UNASSIGNED: In this study, we identified the NBS-LRR genes of 23 representative species at whole genome level, and researches on NBS-LRR genes of four monocotyledonous grass species, Saccharum spontaneum, Saccharum officinarum, Sorghum bicolor and Miscanthus sinensis, were focused.
UNASSIGNED: We found that whole genome duplication, gene expansion, and allele loss could be factors affecting the number of NBS-LRR genes in the species, and whole genome duplication is likely to be the main cause of the number of NBS-LRR genes in sugarcane. Meanwhile, we also found a progressive trend of positive selection on NBS-LRR genes. These studies further elucidated the evolutionary pattern of NBS-LRR genes in plants. Transcriptome data from multiple sugarcane diseases revealed that more differentially expressed NBS-LRR genes were derived from S. spontaneum than from S. officinarum in modern sugarcane cultivars, and the proportion was significantly higher than the expected. This finding reveals that S. spontaneum has a greater contribution to disease resistance for modern sugarcane cultivars. In addition, we observed allelespecific expression of seven NBS-LRR genes under leaf scald, and 125 NBS-LRR genes responding to multiple diseases were identified. Finally, we built a plant NBS-LRR gene database to facilitate subsequent analysis and use of NBSLRR genes obtained here. In conclusion, this study complemented and completed the research of plant NBS-LRR genes, and discussed how NBS-LRR genes responding to sugarcane diseases, which provided a guide and genetic resources for further research and utilization of NBS-LRR genes.
摘要：
未经批准：在植物进化过程中，核苷酸结合位点（NBS）和富含亮氨酸的重复（LRR）基因对植物抗病性做出了重要贡献。随着许多高质量的植物基因组测序，在全基因组水平上鉴定和全面分析NBS-LRR基因对于理解和利用它们具有重要意义。
未经批准：在这项研究中，我们在全基因组水平上鉴定了23个代表性物种的NBS-LRR基因，4种单子叶草NBS-LRR基因的研究,糖精,甘蔗,双色高粱和芒草，集中。
未经证实：我们发现全基因组重复，基因扩增,等位基因丢失可能是影响物种中NBS-LRR基因数量的因素，而全基因组复制可能是甘蔗NBS-LRR基因数量增加的主要原因。同时,我们还发现NBS-LRR基因的阳性选择呈渐进趋势。这些研究进一步阐明了NBS-LRR基因在植物中的进化模式。来自多种甘蔗疾病的转录组数据显示，在现代甘蔗品种中，自发性S.spontaeum比来自S.officinarum的差异表达NBS-LRR基因更多。且比例明显高于预期。这一发现表明，自发性链球菌对现代甘蔗品种的抗病性有更大的贡献。此外,我们观察到7个NBS-LRR基因在叶片烫伤下的等位基因特异性表达，并鉴定了125个对多种疾病有反应的NBS-LRR基因。最后,我们建立了植物NBS-LRR基因数据库，以方便后续分析和使用此处获得的NBSLRR基因。总之,本研究补充并完成了植物NBS-LRR基因的研究,并讨论了NBS-LRR基因如何应对甘蔗病害，为NBS-LRR基因的进一步研究和利用提供了指导和遗传资源。
未经批准：在这项研究中，我们在全基因组水平上鉴定了23个代表性物种的NBS-LRR基因，4种单子叶草NBS-LRR基因的研究,糖精,甘蔗,双色高粱和芒草，集中。
未经证实：我们发现全基因组重复，基因扩增,等位基因丢失可能是影响物种中NBS-LRR基因数量的因素，而全基因组复制可能是甘蔗NBS-LRR基因数量增加的主要原因。同时,我们还发现NBS-LRR基因的阳性选择呈渐进趋势。这些研究进一步阐明了NBS-LRR基因在植物中的进化模式。来自多种甘蔗疾病的转录组数据显示，在现代甘蔗品种中，自发性S.spontaeum比来自S.officinarum的差异表达NBS-LRR基因更多。且比例明显高于预期。这一发现表明，自发性链球菌对现代甘蔗品种的抗病性有更大的贡献。此外,我们观察到7个NBS-LRR基因在叶片烫伤下的等位基因特异性表达，并鉴定了125个对多种疾病有反应的NBS-LRR基因。最后,我们建立了植物NBS-LRR基因数据库，以方便后续分析和使用此处获得的NBSLRR基因。总之,本研究补充并完成了植物NBS-LRR基因的研究,并讨论了NBS-LRR基因如何应对甘蔗病害，为NBS-LRR基因的进一步研究和利用提供了指导和遗传资源。
