PDF(Pubmed)
Abstract：
Sampling of the nasal epithelial lining fluid is a potential method to assess exposure to air pollution within the respiratory tract among high risk populations. We investigated associations of short- and long-term particulate matter exposure (PM) and pollution-related metals in the nasal fluid of people with chronic obstructive pulmonary disease (COPD). This study included 20 participants with moderate-to-severe COPD from a larger study who measured long-term personal exposure to PM2.5 using portable air monitors and short-term PM2.5 and black carbon (BC) using in-home samplers for the seven days preceding nasal fluid collection. Nasal fluid was sampled from both nares by nasosorption, and inductively coupled plasma mass spectrometry was used to determine the concentration of metals with major airborne sources. Correlations of selected elements (Fe, Ba, Ni, Pb, V, Zn, Cu) were determined within the nasal fluid. Associations between personal long-term PM2.5 and seven day home PM2.5 and BC exposure and nasal fluid metal concentrations were determined by linear regression. Within nasal fluid samples, concentrations of vanadium and nickel (r = 0.8) and lead and zinc (r = 0.7) were correlated. Seven day and long-term PM2.5 exposure were both associated with higher levels of copper, lead, and vanadium in the nasal fluid. BC exposure was associated with higher levels of nickel in the nasal fluid. Levels of certain metals in the nasal fluid may serve as biomarkers of air pollution exposure in the upper respiratory tract.
摘要：
鼻上皮衬里液的采样是评估高危人群中呼吸道空气污染暴露的潜在方法。我们调查了慢性阻塞性肺疾病（COPD）患者鼻液中短期和长期颗粒物暴露（PM）与污染相关金属的关系。这项研究包括来自一项较大研究的20名中度至重度COPD参与者，他们使用便携式空气监测仪测量了长期个人暴露于PM2.5，并使用家用采样器测量了短期PM2.5和黑碳（BC）。鼻液收集前7天。通过鼻吸附从两个鼻孔取样鼻液，电感耦合等离子体质谱法用于确定主要空气源的金属浓度。所选元素的相关性(Fe，Ba,Ni,Pb,V,Zn,Cu）在鼻液中测定。通过线性回归确定个人长期PM2.5与7天家庭PM2.5和BC暴露与鼻液金属浓度之间的关联。在鼻液样本中，钒和镍（r=0.8）与铅和锌（r=0.7）的浓度相关。7天和长期的PM2.5暴露都与较高的铜含量有关，铅,还有鼻液中的钒.BC暴露与鼻液中镍含量较高有关。鼻液中某些金属的水平可以作为上呼吸道空气污染暴露的生物标志物。
