METHODS: We performed cultures of stool samples from healthcare workers in unit A and unit B (another unit without SGSP sepsis). If SGSP was positive in feces, we performed isolate pulsotyping and genotyping by using pulsed-field gel electrophoresis (PFGE) and analyzing random amplified polymorphic DNA (RAPD) patterns, respectively.
RESULTS: Five staff members in unit A showed positivity for SGSP. All samples from unit B were negative. We identified two major pulsogroups (groups C and D) by PFGE. In group D, the strains isolated from 3 consecutive sepsis patients (P1, P2 and P3) were closely related and clustered together as those from 2 staff members (C1/C2, C6). One staff (staff 4) had a direct contact history with patient (P1) confirmed to have the same clone. The last isolate of the patient in our study (P4) belonged to a distinct clone.
CONCLUSIONS: We found prolonged gut colonization of SGSP in healthcare workers and its epidemiological relatedness to neonatal sepsis. Fecal-oral or contact transmission is a possible route of SGSP infection. Fecal shedding among staff may be associated with neonatal sepsis in healthcare facilities.
方法:我们对A单元和B单元(另一个没有SGSP脓毒症的单元)的医护人员的粪便样本进行了培养。如果SGSP在粪便中呈阳性,我们通过使用脉冲场凝胶电泳(PFGE)和分析随机扩增多态性DNA(RAPD)模式进行分离脉冲分型和基因分型,分别。
结果:A单元的五名工作人员对SGSP呈阳性。来自单元B的所有样品均为阴性。我们通过PFGE确定了两个主要的脉冲组(组C和D)。D组,从3例连续脓毒症患者(P1,P2和P3)中分离出的菌株与2例工作人员(C1/C2,C6)中的菌株密切相关并聚集在一起.一名工作人员(工作人员4)与患者(P1)有直接接触史,证实具有相同的克隆。我们研究中患者的最后一个分离株(P4)属于一个独特的克隆。
结论:我们发现SGSP在医护人员中的肠道定植时间延长,其与新生儿败血症的流行病学相关性。粪便-口腔或接触性传播是SGSP感染的可能途径。工作人员的粪便脱落可能与医疗机构中的新生儿败血症有关。