Abstract：
BACKGROUND: Streptococcus gallolyticus subsp. pasteurianus (SGSP) is a commensal in the intestinal tract and a potential pathogen of neonatal sepsis. During an 11-month period, four consecutive cases of SGSP sepsis were identified in one postnatal care unit (unit A) without evidence of vertical transmission. Therefore, we initiated this study to investigate the reservoir and mode of transmission of SGSP.
METHODS: We performed cultures of stool samples from healthcare workers in unit A and unit B (another unit without SGSP sepsis). If SGSP was positive in feces, we performed isolate pulsotyping and genotyping by using pulsed-field gel electrophoresis (PFGE) and analyzing random amplified polymorphic DNA (RAPD) patterns, respectively.
RESULTS: Five staff members in unit A showed positivity for SGSP. All samples from unit B were negative. We identified two major pulsogroups (groups C and D) by PFGE. In group D, the strains isolated from 3 consecutive sepsis patients (P1, P2 and P3) were closely related and clustered together as those from 2 staff members (C1/C2, C6). One staff (staff 4) had a direct contact history with patient (P1) confirmed to have the same clone. The last isolate of the patient in our study (P4) belonged to a distinct clone.
CONCLUSIONS: We found prolonged gut colonization of SGSP in healthcare workers and its epidemiological relatedness to neonatal sepsis. Fecal-oral or contact transmission is a possible route of SGSP infection. Fecal shedding among staff may be associated with neonatal sepsis in healthcare facilities.
METHODS: We performed cultures of stool samples from healthcare workers in unit A and unit B (another unit without SGSP sepsis). If SGSP was positive in feces, we performed isolate pulsotyping and genotyping by using pulsed-field gel electrophoresis (PFGE) and analyzing random amplified polymorphic DNA (RAPD) patterns, respectively.
RESULTS: Five staff members in unit A showed positivity for SGSP. All samples from unit B were negative. We identified two major pulsogroups (groups C and D) by PFGE. In group D, the strains isolated from 3 consecutive sepsis patients (P1, P2 and P3) were closely related and clustered together as those from 2 staff members (C1/C2, C6). One staff (staff 4) had a direct contact history with patient (P1) confirmed to have the same clone. The last isolate of the patient in our study (P4) belonged to a distinct clone.
CONCLUSIONS: We found prolonged gut colonization of SGSP in healthcare workers and its epidemiological relatedness to neonatal sepsis. Fecal-oral or contact transmission is a possible route of SGSP infection. Fecal shedding among staff may be associated with neonatal sepsis in healthcare facilities.
摘要：
背景：溶胆链球菌亚种。巴氏菌（SGSP）是肠道中的共生菌，是新生儿败血症的潜在病原体。在11个月的时间里，在一个产后护理单元(A单元)中发现了4例SGSP脓毒症连续病例,没有垂直传播的证据.因此,我们启动了这项研究，以调查SGSP的储层和传播方式。
方法：我们对A单元和B单元（另一个没有SGSP脓毒症的单元）的医护人员的粪便样本进行了培养。如果SGSP在粪便中呈阳性,我们通过使用脉冲场凝胶电泳（PFGE）和分析随机扩增多态性DNA（RAPD）模式进行分离脉冲分型和基因分型，分别。
结果：A单元的五名工作人员对SGSP呈阳性。来自单元B的所有样品均为阴性。我们通过PFGE确定了两个主要的脉冲组(组C和D)。D组,从3例连续脓毒症患者(P1,P2和P3)中分离出的菌株与2例工作人员(C1/C2,C6)中的菌株密切相关并聚集在一起.一名工作人员（工作人员4）与患者（P1）有直接接触史，证实具有相同的克隆。我们研究中患者的最后一个分离株（P4）属于一个独特的克隆。
结论：我们发现SGSP在医护人员中的肠道定植时间延长，其与新生儿败血症的流行病学相关性。粪便-口腔或接触性传播是SGSP感染的可能途径。工作人员的粪便脱落可能与医疗机构中的新生儿败血症有关。
方法：我们对A单元和B单元（另一个没有SGSP脓毒症的单元）的医护人员的粪便样本进行了培养。如果SGSP在粪便中呈阳性,我们通过使用脉冲场凝胶电泳（PFGE）和分析随机扩增多态性DNA（RAPD）模式进行分离脉冲分型和基因分型，分别。
结果：A单元的五名工作人员对SGSP呈阳性。来自单元B的所有样品均为阴性。我们通过PFGE确定了两个主要的脉冲组(组C和D)。D组,从3例连续脓毒症患者(P1,P2和P3)中分离出的菌株与2例工作人员(C1/C2,C6)中的菌株密切相关并聚集在一起.一名工作人员（工作人员4）与患者（P1）有直接接触史，证实具有相同的克隆。我们研究中患者的最后一个分离株（P4）属于一个独特的克隆。
结论：我们发现SGSP在医护人员中的肠道定植时间延长，其与新生儿败血症的流行病学相关性。粪便-口腔或接触性传播是SGSP感染的可能途径。工作人员的粪便脱落可能与医疗机构中的新生儿败血症有关。
