Abstract：
During early vertebrate development, signals from a special region of the embryo, the organizer, can redirect the fate of non-neural ectoderm cells to form a complete, patterned nervous system. This is called neural induction and has generally been imagined as a single signalling event, causing a switch of fate. Here, we undertake a comprehensive analysis, in very fine time course, of the events following exposure of competent ectoderm of the chick to the organizer (the tip of the primitive streak, Hensen\'s node). Using transcriptomics and epigenomics we generate a gene regulatory network comprising 175 transcriptional regulators and 5614 predicted interactions between them, with fine temporal dynamics from initial exposure to the signals to expression of mature neural plate markers. Using in situ hybridization, single-cell RNA-sequencing, and reporter assays, we show that the gene regulatory hierarchy of responses to a grafted organizer closely resembles the events of normal neural plate development. The study is accompanied by an extensive resource, including information about conservation of the predicted enhancers in other vertebrates.
摘要：
在脊椎动物早期发育过程中,来自胚胎的特殊区域的信号，组织者,可以改变非神经外胚层细胞的命运，形成一个完整的，有图案的神经系统.这被称为神经感应，通常被认为是一个单一的信号事件，导致命运的转变。这里,我们进行了全面的分析，在非常好的时间过程中，小鸡有能力的外胚层暴露于组织者(原始条纹的尖端，Hensen\的节点)。使用转录组学和表观基因组学，我们生成了一个基因调控网络，包括175个转录调控因子和5614个预测的它们之间的相互作用。从最初暴露于信号到成熟神经板标记表达的精细时间动态。使用原位杂交，单细胞RNA测序，和报告分析，我们表明，对嫁接组织者的反应的基因调节层次与正常神经板发育的事件非常相似。这项研究伴随着广泛的资源，包括有关其他脊椎动物中预测的增强子保守性的信息。
