Abstract：
BACKGROUND: Feline Panleukopenia is an important disease of cats and has been reported worldwide. The disease is caused by a non-enveloped, single-stranded DNA virus; Feline Panleukopenia Virus (FPLV), belonging to the Parvoviridae family. The disease causes significant mortality in unvaccinated kittens. The disease has been well documented in companion animals. However, only a few reports have surfaced from the wild.
METHODS: An orphan leopard cub was presented to Wildlife Rescue Centre, Nagpur, for further care; the leopard was kept under quarantine. On day 22 of the quarantine, the leopard showed inappetence, lethargy and depression and did not consume the offered carabeef (Day 0 of treatment). The leopard was examined clinically and was found to have a temperature of 102°F; blood was collected and analysed. On day one, the leopard exhibited bloody diarrhoea, inappetence, fever and depression. The leopard was rationally treated with fluids, antibiotics, multi-vitamins, haemostatics and haematinics. To gain qualitative insights into the epidemiological aspect of the disease, molecular investigation, including Polymerase Chain Reaction (PCR) and qPCR (Quantitative Polymerase Chain Reaction), were utilized to confirm the infection. The amplicon was sequenced and was found to be similar to sequences of FPLV reported domestic cats and other wild felids from India and abroad. Phylogenetic analysis was performed to understand the evolutionary relationship of the virus with previously reported sequences of FPLV. Sequences were submitted to National Center for Biotechnology Information (NCBI) and were allotted accession numbers.
CONCLUSIONS: The infection in endangered leopard cubs could be managed with prompt fluid therapy, antibiotics and support treatment, ensuring an uneventful recovery. Molecular investigation and sequencing efforts can provide valuable data on epidemiology and the evolutionary relationship of the virus with the circulating strains in the field. The study has implications in the preventive management of FPLV in captivity and the selection of strains for inclusion in vaccines meant for the wild felids.
METHODS: An orphan leopard cub was presented to Wildlife Rescue Centre, Nagpur, for further care; the leopard was kept under quarantine. On day 22 of the quarantine, the leopard showed inappetence, lethargy and depression and did not consume the offered carabeef (Day 0 of treatment). The leopard was examined clinically and was found to have a temperature of 102°F; blood was collected and analysed. On day one, the leopard exhibited bloody diarrhoea, inappetence, fever and depression. The leopard was rationally treated with fluids, antibiotics, multi-vitamins, haemostatics and haematinics. To gain qualitative insights into the epidemiological aspect of the disease, molecular investigation, including Polymerase Chain Reaction (PCR) and qPCR (Quantitative Polymerase Chain Reaction), were utilized to confirm the infection. The amplicon was sequenced and was found to be similar to sequences of FPLV reported domestic cats and other wild felids from India and abroad. Phylogenetic analysis was performed to understand the evolutionary relationship of the virus with previously reported sequences of FPLV. Sequences were submitted to National Center for Biotechnology Information (NCBI) and were allotted accession numbers.
CONCLUSIONS: The infection in endangered leopard cubs could be managed with prompt fluid therapy, antibiotics and support treatment, ensuring an uneventful recovery. Molecular investigation and sequencing efforts can provide valuable data on epidemiology and the evolutionary relationship of the virus with the circulating strains in the field. The study has implications in the preventive management of FPLV in captivity and the selection of strains for inclusion in vaccines meant for the wild felids.
摘要：
背景：猫泛白细胞减少症是猫的一种重要疾病，已在世界范围内得到报道。这种疾病是由无包膜引起的，单链DNA病毒;猫泛白细胞减少症病毒,属于细小病毒科。该疾病在未接种疫苗的小猫中导致显著的死亡率。这种疾病已经在伴侣动物中得到了很好的证明。然而,只有少数报告从野外浮出水面。
方法：一只孤儿豹幼崽被送到野生动物救援中心，那格浦尔,为了进一步的护理；豹子被隔离。在隔离的第22天,豹子表现出食欲不振，嗜睡和抑郁，不食用所提供的carabef（治疗第0天）。对豹子进行了临床检查，发现温度为102°F；收集血液并进行分析。第一天,豹子表现出血腥的腹泻，食欲不振,发烧和抑郁豹子被合理地处理了液体，抗生素,多种维生素,止血药和止血药。为了获得对疾病流行病学方面的定性见解，分子调查，包括聚合酶链反应（PCR）和qPCR（定量聚合酶链反应），被用来确认感染。对扩增子进行测序，发现与FPLV报道的印度和国外的家猫和其他野生猫科动物的序列相似。进行系统发育分析以了解病毒与先前报道的FPLV序列的进化关系。将序列提交给国家生物技术信息中心(NCBI)并分配登录号。
结论：可以通过及时的液体治疗来控制濒危豹子幼崽的感染，抗生素和支持治疗，确保顺利恢复。分子调查和测序工作可以提供有关流行病学和病毒与该领域中的流行株的进化关系的有价值的数据。该研究对圈养FPLV的预防性管理以及选择用于野生猫科动物的疫苗中的菌株具有重要意义。
方法：一只孤儿豹幼崽被送到野生动物救援中心，
结论：可以通过及时的液体治疗来控制濒危豹子幼崽的感染，
