PDF(Pubmed)
Abstract：
The current study examined the phylogenetic pattern of medicinal species of the family Apiaceae based on flavonoid groups production, as well as the overall mechanism of the key genes involved in flavonol and flavone production. Thirteen species of the family Apiaceae were used, including Eryngium campestre from the subfamily Saniculoideae, as well as Cuminum cyminum, Carum carvi, Coriandrum sativum, Apium graveolens, Petroselinum crispum, Pimpinella anisum, Anethum graveolens, Foeniculum vulgare, Daucus carota, Ammi majus, Torilis arvensis, and Deverra tortuosa from the subfamily Apioideae. The seeds were cultivated, and the leaves were collected to estimate flavonoids and their groups, physiological factors, transcription levels of flavonol and flavone production-related genes. The phylogenetic relationship between the studied species was established using the L-ribosomal 16 (rpl16) chloroplast gene. The results revealed that the studied species were divided into two patterns: six plant species, E. campestre, C. carvi, C. sativum, P. anisum, An. graveolens, and D. carota, contained low content of flavonoids, while the other seven species had high content. This pattern of flavonoids production coincided with the phylogenetic relationships between the studied species. In contrast, the phylogeny of the flavonol and flavone synthase genes was incompatible with the quantitative production of their products. The study concluded that the increment in the production of flavonol depends on the high expression of chalcone synthase, chalcone isomerase, flavanone 3 hydroxylase, flavonol synthase, the increase of Abscisic acid, sucrose, and phenyl ammonia lyase, while flavone mainly depends on evolution and on the high expression of the flavone synthase gene.
摘要：
当前的研究基于类黄酮基团的产生，研究了伞形科药用物种的系统发育模式，以及黄酮醇和黄酮生产中涉及的关键基因的整体机制。使用了13种菊科植物，包括来自Saniculoideae亚科的桉树，以及CuminumCuminumCuminum,CarumCarvi,科里兰姆，罂粟，石油脆片，阿尼森皮普诺拉,Anethumgraveolens,小鹿，DaucusCarota,Ammimajus，Torilisarvensis,和Apioideae亚科的DeverraTortuosa。种植的种子，收集叶子来估计黄酮类化合物及其组，生理因素,黄酮醇和黄酮生产相关基因的转录水平。使用L-核糖体16（rpl16）叶绿体基因建立了所研究物种之间的系统发育关系。结果表明，研究的物种分为两种模式：六种植物，E.Campestre,C.Carvi,C.sativum,P.Anisum,A.graveolens,还有D.Carota,含有低含量的类黄酮，而其他七个物种含量较高。这种类黄酮生产模式与所研究物种之间的系统发育关系相吻合。相比之下,黄酮醇和黄酮合酶基因的系统发育与其产品的定量生产不相容。该研究得出结论，黄酮醇产量的增加取决于查尔酮合酶的高表达，查尔酮异构酶，黄烷酮3羟化酶，黄酮醇合成酶，脱落酸的增加，蔗糖,和苯基氨裂解酶，而黄酮主要取决于进化和黄酮合酶基因的高表达。
