PDF(Pubmed)
Abstract：
UNASSIGNED: Grape rootstocks play critical role in the development of the grape industry over the globe for their higher adaptability to various environments, and the evaluation of their genetic diversity among grape genotypes is necessary to the conservation and utility of genotypes.
UNASSIGNED: To analyze the genetic diversity of grape rootstocks for a better understanding multiple resistance traits, whole-genome re-sequencing of 77 common grape rootstock germplasms was conducted in the present study.
UNASSIGNED: About 645 billion genome sequencing data were generated from the 77 grape rootstocks at an average depth of ~15.5×, based on which the phylogenic clusters were generated and the domestication of grapevine rootstocks was explored. The results indicated that the 77 rootstocks originated from five ancestral components. Through phylogenetic, principal components, and identity-by-descent (IBD) analyses, these 77 grape rootstocks were assembled into ten groups. It is noticed that the wild resources of V. amurensis and V. davidii, originating from China and being generally considered to have stronger resistance against biotic and abiotic stresses, were sub-divided from the other populations. Further analysis indicated that a high level of linkage disequilibrium was found among the 77 rootstock genotypes, and a total of 2,805,889 single nucleotide polymorphisms (SNPs) were excavated, GWAS analysis among the grape rootstocks located 631, 13, 9, 2, 810, and 44 SNP loci that were responsible to resistances to phylloxera, root-knot nematodes, salt, drought, cold and waterlogging traits.
UNASSIGNED: This study generated a significant amount of genomic data from grape rootstocks, thus providing a theoretical basis for further research on the resistance mechanism of grape rootstocks and the breeding of resistant varieties. These findings also reveal that China originated V. amurensis and V. davidii could broaden the genetic background of grapevine rootstocks and be important germplasm used in breeding high stress-resistant grapevine rootstocks.
UNASSIGNED: To analyze the genetic diversity of grape rootstocks for a better understanding multiple resistance traits, whole-genome re-sequencing of 77 common grape rootstock germplasms was conducted in the present study.
UNASSIGNED: About 645 billion genome sequencing data were generated from the 77 grape rootstocks at an average depth of ~15.5×, based on which the phylogenic clusters were generated and the domestication of grapevine rootstocks was explored. The results indicated that the 77 rootstocks originated from five ancestral components. Through phylogenetic, principal components, and identity-by-descent (IBD) analyses, these 77 grape rootstocks were assembled into ten groups. It is noticed that the wild resources of V. amurensis and V. davidii, originating from China and being generally considered to have stronger resistance against biotic and abiotic stresses, were sub-divided from the other populations. Further analysis indicated that a high level of linkage disequilibrium was found among the 77 rootstock genotypes, and a total of 2,805,889 single nucleotide polymorphisms (SNPs) were excavated, GWAS analysis among the grape rootstocks located 631, 13, 9, 2, 810, and 44 SNP loci that were responsible to resistances to phylloxera, root-knot nematodes, salt, drought, cold and waterlogging traits.
UNASSIGNED: This study generated a significant amount of genomic data from grape rootstocks, thus providing a theoretical basis for further research on the resistance mechanism of grape rootstocks and the breeding of resistant varieties. These findings also reveal that China originated V. amurensis and V. davidii could broaden the genetic background of grapevine rootstocks and be important germplasm used in breeding high stress-resistant grapevine rootstocks.
摘要：
未经评估：葡萄砧木因其对各种环境的更高适应性，在全球葡萄产业的发展中起着至关重要的作用。葡萄基因型之间的遗传多样性评估对于基因型的保存和利用是必要的。
UNASSIGNED：为了分析葡萄砧木的遗传多样性，以更好地了解多种抗性性状，本研究对77份普通葡萄砧木种质进行了全基因组重测序。
UNASSIGNED：从平均深度约15.5×的77个葡萄砧木中产生了约6450亿个基因组测序数据，在此基础上产生了系统发育簇，并探索了葡萄砧木的驯化。结果表明，77个砧木起源于五种祖先成分。通过系统发育，主要成分，和血统身份(IBD)分析，这77个葡萄砧木被组装成十组。值得注意的是，阿穆rensis和V.davidii的野生资源，原产于中国，通常被认为对生物和非生物胁迫具有更强的抗性，从其他人群中细分。进一步的分析表明，在77种砧木基因型中发现了高水平的连锁不平衡，共挖掘2,805,889个单核苷酸多态性(SNPs),葡萄砧木中的GWAS分析位于631、13、9、2、810和44个SNP基因座，这些SNP基因座负责对根瘤菌的抗性，根结线虫,盐,干旱,寒冷和内涝特征。
未经评估：这项研究从葡萄砧木中获得了大量的基因组数据，为进一步研究葡萄砧木的抗性机制和选育抗病性品种提供了理论依据。这些发现还表明，中国原产的V.amurensis和V.davidii可以拓宽葡萄砧木的遗传背景，是选育高抗逆性葡萄砧木的重要种质。
UNASSIGNED：为了分析葡萄砧木的遗传多样性，以更好地了解多种抗性性状，
UNASSIGNED：从平均深度约15.5×的77个葡萄砧木中产生了约6450亿个基因组测序数据，
未经评估：这项研究从葡萄砧木中获得了大量的基因组数据，
