Abstract：
Introduction: To explore the value of serum sirtuin-1 (SIRT1) in the diagnosis and evaluation of joint mobility of rheumatoid arthritis (RA). Materials and Methods: Serum was randomly obtained from 212 RA patients,210 non-RA patients and 58 healthy controls in a large tertiary first-class hospital in Jiangxi province from November 2021 to June 2022. The level of serum Sirt1,anti-cyclic citrulline polypeptide antibody (anti-CCP), anti-mutant citrulline vimentin antibody (anti-MCV), rheumatoid factor (RF),high-mobility group box 1 (HMGB1), collagen triple helix repeat containing 1 (CTHRC1), erythrocyte sedimentation rate (ESR) and C-reactive protein (CRP) were detected by ELISA, to explore the correlation between them and their value in the diagnosis and evaluation of joint range of motion of RA and statistically analyse their diagnostic efficiency. Results: ① The level of all markers was higher in the RA group than in the non-RA group and the healthy controls (p < 0.05). ② The AUC of the SIRT1 was 0.882, second only to the anti-MCV and anti-CCP. ③ The anti-CCP showed the highest sensitivity to RA diagnosis of 0.948. The specificity and positive predictive value of SIRT1 for the diagnosis of RA were the highest, which are 0.959 and 0.934 respectively. ④ In serial combination, SIRT1/anti-CCP、SIRT1/anti-MCV showed the highest specificity.SIRT1/anti-CCP in parallel combination had the highest sensitivity. ⑤ SIRT1 showed a significant correlation with other markers and DAS28 scores (p < 0.01). Conclusion: SIRT1 can be used as a new serological marker for RA diagnosis, which has a significant correlation with RA joint mobility and has a certain reference value in RA differential diagnosis, providing a new detection basis for RA differential diagnosis.
摘要：
目的：探讨血清sirtuin-1（SIRT1）在类风湿关节炎（RA）关节活动度诊断和评估中的价值。材料和方法：从212例RA患者中随机获取血清，2021年11月至2022年6月，江西省某大型三级甲等医院210例非RA患者和58例健康对照。血清Sirt1、抗环瓜氨酸多肽抗体(抗CCP)、抗突变型瓜氨酸波形蛋白抗体（抗MCV），类风湿因子（RF），高移动性组盒1(HMGB1)，含胶原蛋白三螺旋重复1（CTHRC1），ELISA法检测血沉(ESR)和C反应蛋白(CRP),探讨它们之间的相关性及其在RA诊断和评估关节活动度中的价值，并对其诊断效率进行统计学分析。结果:①RA组各项指标水平均高于非RA组和健康对照组(p<0.05)。②SIRT1的AUC为0.882，仅次于抗MCV和抗CCP。③抗CCP对RA诊断的敏感性最高，为0.948。SIRT1诊断RA的特异性和阳性预测值最高，分别为0.959和0.934。④串行组合，SIRT1/抗CCP、SIRT1/抗MCV特异性最高。SIRT1/抗CCP的平行组合具有最高的灵敏度。⑤SIRT1与其他标志物及DAS28评分呈显著相关(p<0.01)。结论:SIRT1可作为RA诊断的新血清学标志物,与RA关节活动度有显著的相关性,对RA的鉴别诊断有一定的参考价值,为RA的鉴别诊断提供了新的检测依据。
