关键词: MEOX2 adipogenesis circINSR miR-152 sheep stromal vascular fraction

Mesh : Sheep / genetics Animals MicroRNAs / genetics Stromal Vascular Fraction Adipose Tissue / metabolism Adipogenesis / genetics Lipid Metabolism Cell Differentiation

来  源:   DOI:10.3390/ijms24043501

Abstract:
Adipose tissue plays a crucial role in energy metabolism. Several studies have shown that circular RNA (circRNA) is involved in the regulation of fat development and lipid metabolism. However, little is known about their involvement in the adipogenic differentiation of ovine stromal vascular fractions (SVFs). Here, based on previous sequencing data and bioinformatics analysis, a novel circINSR was identified in sheep, which acts as a sponge to promote miR-152 in inhibiting the adipogenic differentiation of ovine SVFs. The interactions between circINSR and miR-152 were examined using bioinformatics, luciferase assays, and RNA immunoprecipitation. Of note, we found that circINSR was involved in adipogenic differentiation via the miR-152/mesenchyme homeobox 2 (MEOX2) pathway. MEOX2 inhibited adipogenic differentiation of ovine SVFs and miR-152 inhibited the expression of MEOX2. In other words, circINSR directly isolates miR-152 in the cytoplasm and inhibits its ability to promote adipogenic differentiation of ovine SVFs. In summary, this study revealed the role of circINSR in the adipogenic differentiation of ovine SVFs and its regulatory mechanisms, providing a reference for further interpretation of the development of ovine fat and its regulatory mechanisms.
摘要:
脂肪组织在能量代谢中起着至关重要的作用。多项研究表明,环状RNA(circularRNA,circRNA)参与脂肪发育和脂质代谢的调节。然而,关于它们参与绵羊基质血管部分(SVFs)的成脂分化知之甚少。这里,基于以前的测序数据和生物信息学分析,在绵羊身上发现了一部小说,它作为海绵促进miR-152抑制绵羊SVFs的成脂分化。使用生物信息学检查了circINSR和miR-152之间的相互作用,荧光素酶测定,和RNA免疫沉淀。值得注意的是,我们发现circINSR通过miR-152/间充质同源异型盒2(MEOX2)通路参与脂肪分化.MEOX2抑制绵羊SVFs的成脂分化,miR-152抑制MEOX2的表达。换句话说,circINSR直接在细胞质中分离miR-152并抑制其促进绵羊SVF成脂分化的能力.总之,这项研究揭示了circINSR在绵羊SVFs成脂分化中的作用及其调控机制,为进一步解释绵羊脂肪的发育及其调控机制提供参考。
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