Abstract：
Marker-assisted selection is an important method for livestock breeding. In recent years, this technology has been gradually applied to livestock breeding to improve the body conformation traits. In this study, the LRRC8B (Leucine Rich Repeat Containing 8 VRAC Subunit B) gene was selected to evaluate the association between its genetic variations and the body conformation traits in two native sheep breeds in China. Four body conformation traits, including withers height, body length, chest circumference, and body weight, were collected from 269 Chaka sheep. We also collected the body length, chest width, withers height, chest depth, chest circumference, cannon bone circumference, and height at hip cross of 149 Small-Tailed Han sheep. Two different genotypes, ID and DD, were detected in all sheep. Our data showed that the polymorphism of the LRRC8B gene was significantly associated with chest depth (p < 0.05) in Small-Tailed Han sheep, and it is greater in sheep with DD than those with ID. In conclusion, our data suggested that the LRRC8B gene could serve as a candidate gene for marker-assisted selection in Small-Tailed Han sheep.
摘要：
标记辅助选择是家畜育种的重要方法。近年来,该技术已逐步应用于家畜育种，以改善机体构象性状。在这项研究中,选择LRRC8B（含8个VRAC亚基B的亮氨酸丰富重复序列）基因，以评估其遗传变异与中国两个本地绵羊品种的身体构象性状之间的关联。四个身体构象特征，包括枯萎高度，身体长度,胸围,和体重，是从269只Chaka绵羊中收集的。我们还收集了身体长度，胸部宽度,枯萎的高度，胸部深度,胸围,大炮骨围,149只小尾寒羊的臀部交叉高度。两种不同的基因型,ID和DD,在所有绵羊身上都被发现。我们的数据表明，LRRC8B基因的多态性与小尾寒羊的胸部深度显着相关（p<0.05），DD的绵羊比ID的绵羊更大。总之,我们的数据表明,LRRC8B基因可以作为小尾寒羊标记辅助选择的候选基因.
