PDF(Pubmed)
Abstract：
Despite the increased interest in macroalgae protein and fibers, little information is available on their bioaccessibility. The application of an in vitro gastrointestinal digestion model to study the degree of disintegration and release of proteins with expressed bioactivities from wild and cultivated Palmaria palmata and Saccharina latissima was proposed in this study. Macroalgae from the Gulf of St Lawrence, Canada, were submitted to digestive transit times of 2 (oral), 60 (gastric) and 120 (duodenal) minutes. Among wild samples, P. palmata had a higher percentage of disintegration, protein release and degree of hydrolysis than S. latissima. While the least digested sample, wild S. latissima, was the sample with the highest antioxidant activity (210 μmol TE g-1), the most digested sample, cultivated P. palmata, presented the highest ability to inhibit the angiotensin-converting enzyme (ACE), reaching 32.6 ± 1.2% at 3 mg mL-1. ACE inhibitory activity increased from 1 to 3 mg mL-1, but not at 5 mg mL-1. Wild samples from both species showed an ACE inhibition around 27.5%. Data suggested that the disintegration of the samples was influenced by their soluble and insoluble fiber contents. Further information on the bioaccessibility and bioactivity of these macroalgae should consider the characterization of digestion products other than protein, as well as the effects of previous product processing.
摘要：
尽管人们对巨藻蛋白和纤维的兴趣增加，关于它们的生物可及性的信息很少。本研究提出了应用体外胃肠道消化模型研究野生和栽培的Palmariapalmata和Saccharinalatissima中具有表达生物活性的蛋白质的崩解和释放程度。来自圣劳伦斯湾的大型藻类，加拿大,接受2次（口服）的消化运输时间，60（胃）和120（十二指肠）分钟。在野生样本中，P.palmata的崩解比例更高，蛋白质释放和水解度比拉氏链球菌。虽然消化最少的样本，野生S.latissima，是抗氧化活性最高的样品(210μmolTEg-1)，消化最多的样本，栽培的P.palmata,表现出最高的抑制血管紧张素转换酶（ACE）的能力，在3mgmL-1时达到32.6±1.2％。ACE抑制活性从1mgmL-1增加到3mgmL-1，但在5mgmL-1时没有增加。来自两个物种的野生样品显示约27.5%的ACE抑制。数据表明，样品的崩解受其可溶性和不溶性纤维含量的影响。有关这些大型藻类的生物可及性和生物活性的进一步信息应考虑除蛋白质以外的消化产物的表征，以及以前产品加工的影响。
