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Abstract：
UNASSIGNED: To explore the role of sclerosteosis (SOST) gene expression in the occurrence and development of multiple myeloma (MM) complicated with sarcopenia.
UNASSIGNED: Analysis of the SOST expression in skeletal muscle tissue of patients with MM using high-throughput sequencing combined with transcriptomics; observation of morphological changes of the mouse C2C12 myoblasts co-cultured with SP2/0 myeloma cells in Transwell; observation of the SOST expression in the C2C12 myoblasts using the immunofluorescence labeling method; and assessment of the changes in exercise capacity of mice with MM using ethology; and the measurement of the SOST expression in muscles of mice using immunohistochemistry.
UNASSIGNED: The transcription level of the SOST gene in the muscle tissue was significantly higher in patients with MM and sarcopenia than in patients with MM without sarcopenia and elderly patients with sarcopenia; the area of C2C12 mouse myoblasts co-cultured with SP2/0 myeloma cells was 167,904 ± 8653.7 pix; this was significantly lower than the area of 402,994 ± 13,575.0 pix in the control group (CG); the fluorescence intensity of SOST in the cells of the experimental group (EG) was 159,389 ± 10,534 AU; this was significantly higher than the intensity of 26,338 ± 6059 AU in the CG; the differences in results of the coat-hanger test, the tail suspension test, the weight-bearing forced swimming test, and the grip strength test between the tumor-bearing mice in the EG and the CG were statistically significant; and the quantitative result of SOST expression in the muscle tissue of the EG mice was 11,515 ± 1573 pix; this was significantly higher than the result of 3399 ± 798.8 pix in the CG.
UNASSIGNED: The SOST gene expression was significantly higher in muscle of mice in EG than in CG; and increased SOST gene expression might be a pathogenesis of MM complicated with sarcopenia.
UNASSIGNED: Analysis of the SOST expression in skeletal muscle tissue of patients with MM using high-throughput sequencing combined with transcriptomics; observation of morphological changes of the mouse C2C12 myoblasts co-cultured with SP2/0 myeloma cells in Transwell; observation of the SOST expression in the C2C12 myoblasts using the immunofluorescence labeling method; and assessment of the changes in exercise capacity of mice with MM using ethology; and the measurement of the SOST expression in muscles of mice using immunohistochemistry.
UNASSIGNED: The transcription level of the SOST gene in the muscle tissue was significantly higher in patients with MM and sarcopenia than in patients with MM without sarcopenia and elderly patients with sarcopenia; the area of C2C12 mouse myoblasts co-cultured with SP2/0 myeloma cells was 167,904 ± 8653.7 pix; this was significantly lower than the area of 402,994 ± 13,575.0 pix in the control group (CG); the fluorescence intensity of SOST in the cells of the experimental group (EG) was 159,389 ± 10,534 AU; this was significantly higher than the intensity of 26,338 ± 6059 AU in the CG; the differences in results of the coat-hanger test, the tail suspension test, the weight-bearing forced swimming test, and the grip strength test between the tumor-bearing mice in the EG and the CG were statistically significant; and the quantitative result of SOST expression in the muscle tissue of the EG mice was 11,515 ± 1573 pix; this was significantly higher than the result of 3399 ± 798.8 pix in the CG.
UNASSIGNED: The SOST gene expression was significantly higher in muscle of mice in EG than in CG; and increased SOST gene expression might be a pathogenesis of MM complicated with sarcopenia.
摘要：
UNASSIGNED:探讨硬化病(SOST)基因表达在多发性骨髓瘤(MM)合并肌肉减少症发生发展中的作用。
UNASSIGNED：高通量测序结合转录组学分析MM患者骨骼肌组织中SOST的表达；观察Transwell中与SP2/0骨髓瘤细胞共培养的小鼠C2C12成肌细胞的形态学变化；用免疫荧光标记法观察C2C12成肌细胞中SOST的表达；用免疫组织化学测定MM小鼠运动能力
UNASSIGNED：MM和肌肉减少症患者的肌肉组织中SOST基因的转录水平明显高于无肌肉减少症的MM患者和老年肌肉减少症患者；与SP2/0骨髓瘤细胞共培养的C2C12小鼠成肌细胞面积为167,9653.7pix；这在SOpiAU的159,994±CG强度中明显低于对照组的557,9尾部悬挂试验,负重强迫游泳试验,EG和CG中荷瘤小鼠之间的握力测试具有统计学意义；EG小鼠肌肉组织中SOST表达的定量结果为11,515±1573pix；这明显高于CG中3399±798.8pix的结果。
未经证实：EG小鼠肌肉中SOST基因表达明显高于CG；SOST基因表达增加可能是MM并发肌少症的发病机制。
UNASSIGNED：高通量测序结合转录组学分析MM患者骨骼肌组织中SOST的表达；观察Transwell中与SP2/0骨髓瘤细胞共培养的小鼠C2C12成肌细胞的形态学变化；用免疫荧光标记法观察C2C12成肌细胞中SOST的表达；用免疫组织化学测定MM小鼠运动能力
UNASSIGNED：MM和肌肉减少症患者的肌肉组织中SOST基因的转录水平明显高于无肌肉减少症的MM患者和老年肌肉减少症患者；与SP2/0骨髓瘤细胞共培养的C2C12小鼠成肌细胞面积为167,9653.7pix；这在SOpiAU的159,994±CG强度中明显低于对照组的557,9
未经证实：EG小鼠肌肉中SOST基因表达明显高于CG；SOST基因表达增加可能是MM并发肌少症的发病机制。
