PDF(Pubmed)
Abstract：
The human gut virome and its early life development are poorly understood. Prior studies have captured single-point assessments with the evolution of the infant virome remaining largely unexplored. We performed viral metagenomic sequencing on stool samples collected longitudinally from a cohort of 53 infants from age 2 weeks to 3 years (80.7 billion reads), and from their mothers (9.8 billion reads) to examine and compare viromes. The asymptomatic infant virome consisted of bacteriophages, nonhuman dietary/environmental viruses, and human-host viruses, predominantly picornaviruses. In contrast, human-host viruses were largely absent from the maternal virome. Previously undescribed, sequence-divergent vertebrate viruses were detected in the maternal but not infant virome. As infants aged, the phage component evolved to resemble the maternal virome, but by age 3, the human-host component remained dissimilar from the maternal virome. Thus, early life virome development is determined predominantly by dietary, infectious, and environmental factors rather than direct maternal acquisition.
摘要：
人类肠道病毒及其早期生命发展知之甚少。先前的研究已经捕获了单点评估，而婴儿病毒的进化仍未被探索。我们对从2周至3岁的53名婴儿队列中纵向收集的粪便样本进行了病毒宏基因组测序（80.7亿次读数），并从他们的母亲（98亿次阅读）来检查和比较病毒类。无症状的婴儿病毒由噬菌体组成，非人类饮食/环境病毒，和人类宿主病毒，主要是小核糖核酸病毒。相比之下,人类宿主病毒基本上不存在于母体病毒中.以前没有描述过，在母体病毒中检测到序列不同的脊椎动物病毒，但在婴儿病毒中未检测到。随着婴儿年龄的增长，噬菌体成分进化成类似于母体病毒，但是到3岁时，人类宿主成分仍然与母体病毒不同。因此，早期病毒的发展主要由饮食决定，传染性,和环境因素，而不是直接的母亲获得。
