Abstract：
O-specific polysaccharides (O-PSs) isolated from lipopolysaccharides of Serratia spp., strains 10.1WK and 1XS, which are endophytic bacteria of Oenothera biennis (common evening-primrose) and Lotus corniculatus (bird\'s-foot trefoil), plants growing on a petroleum hydrocarbon polluted site in the Silesia region, were investigated. The high-molecular-weight O-PS fractions liberated from lipopolysaccharides by mild acid hydrolysis were studied using chemical methods, MALDI-TOF mass spectrometry, and a set of 1D and 2D NMR spectroscopy techniques. It was found that both O-specific polysaccharides were built of an identical trisaccharide repeating unit composed of d-Rhap and d-Manp residues. The following structure of the O-PSs of Serratia spp. strains 10.1WK and 1XS was established: →4)-α-d-Rhap-(1 → 3)-β-d-Manp-(1 → 4)-β-d-Rhap-(1→.
摘要：
从沙雷氏菌的脂多糖中分离出的O特异性多糖（O-PS）。,菌株10.1WK和1XS，它们是双叶月见草(普通月见草)和莲花(鸟足三叶)的内生细菌，生长在西里西亚地区石油碳氢化合物污染地点的植物，被调查了。使用化学方法研究了通过温和酸水解从脂多糖中释放的高分子量O-PS级分，MALDI-TOF质谱,和一套1D和2DNMR光谱技术。发现两种O特异性多糖均由相同的三糖重复单元构建，该单元由d-Rhap和d-Manp残基组成。沙雷氏菌O-PS的以下结构。建立了10.1WK和1XS菌株：→4）-α-d-Rhap-（1→3）-β-d-Manp-（1→4）-β-d-Rhap-（1→。
