METHODS: Forty-five dental implants were stained with indelible ink and mounted in resin models, which simulated standardised peri-implantitis defects with different bone defect angulations (30°, 60°, and 90°). After each run of instrumentation, the implants were removed from the resin model, and the ink was dissolved in ethanol (97%). A spectrophotometric analysis was performed to detect colour remnants in order to measure the cumulative uncleaned surface area of the implants. Scanning electron microscopy images were taken to assess micromorphological surface changes.
RESULTS: Generally, the 60° bone defects were the easiest to debride, and the 30° defects were the most difficult (ink absorption peak: 0.26±0.04 for 60° defects; 0.32±0.06 for 30° defects; 0.27±0.04 for 90° defects). The most effective debridement method was TiB, independently of the bone defect type (TiB vs. GC: P<0.0001; TiB vs. G-Air: P=0.0017; TiB vs. GE-Air: P=0.0007). GE-Air appeared to be the least efficient method for biofilm debridement.
CONCLUSIONS: T-brushes seem to be a promising decontamination method compared to the other techniques, whereas G-Air was less aggressive on the implant surface. The use of a spectrophotometric model was shown to be a novel but promising assessment method for in vitro ink studies.
方法:将45个牙科植入物用不褪色的墨水染色并安装在树脂模型中,模拟标准化不同骨缺损角度的种植体周围炎缺损(30°,60°,和90°)。每次运行仪器后,从树脂模型中取出植入物,并且将油墨溶解在乙醇(97%)中。进行分光光度分析以检测颜色残留物,以便测量植入物的累积未清洁表面积。获取扫描电子显微镜图像以评估微观形态表面变化。
结果:一般来说,60°骨缺损最容易清除,30°缺陷最困难(油墨吸收峰:60°缺陷为0.26±0.04;30°缺陷为0.32±0.06;90°缺陷为0.27±0.04)。最有效的清创方法是TiB,与骨缺损类型无关(TiBvs.GC:P<0.0001;TiBvs.G-空气:P=0.0017;TiBvs.GE-Air:P=0.0007)。GE-Air似乎是生物膜清创术效率最低的方法。
结论:与其他技术相比,T型刷子似乎是一种有前途的去污方法,而G-Air对植入物表面的侵袭性较小。使用分光光度模型被证明是一种新颖但有前途的体外墨水研究评估方法。