Abstract：
An analytical method using liquid chromatography with tandem mass spectrometry (LC-MS/MS) was established and validated for screening 425 drugs and poisons in dried blood spots (DBSs).
Blood (20 μL) was spotted on Whatman FTA™ classic card to prepare DBS sample, then extracted with 150 μL methanol and analyzed by LC-MS/MS using a multiple reaction monitoring method.
The limit of detection of the compounds were 0.1-10 ng/mL. The values for recovery and matrix effect were 40.3-114.9% and 40.2-118.4%, respectively. This method was successfully applied to DBS samples from 105 humans suspected of drug poisoning, which was stored for 3-5 years at room temperature. Thirty-three kinds of drugs, including benzodiazepines, antipsychotics, antidepressants, antipyretic analgesics, non-steroidal anti-inflammatory drugs, antibiotics, antiepileptic drugs, new psychoactive drugs were confirmed in 102 cases, while no compound was detected in the other 3 cases. Estazolam, a benzodiazepine widely used in clinical practice as a sedative, hypnotic, and anti-anxiety drug, was the most frequently detected substance, occurring in 34.2% of the cases.
Most drugs in DBS could still be detected after storage for 3-5 years, but ambroxol, zopiclone, carbofuran, chlorpyrifos, and valproic acid were not detectable after 3-5 years of storage at room temperature. The components measured in DBS were consistent with those measured in whole blood at the collection time, thereby confirming that DBS samples have the advantage of stable storage at room temperature.
Blood (20 μL) was spotted on Whatman FTA™ classic card to prepare DBS sample, then extracted with 150 μL methanol and analyzed by LC-MS/MS using a multiple reaction monitoring method.
The limit of detection of the compounds were 0.1-10 ng/mL. The values for recovery and matrix effect were 40.3-114.9% and 40.2-118.4%, respectively. This method was successfully applied to DBS samples from 105 humans suspected of drug poisoning, which was stored for 3-5 years at room temperature. Thirty-three kinds of drugs, including benzodiazepines, antipsychotics, antidepressants, antipyretic analgesics, non-steroidal anti-inflammatory drugs, antibiotics, antiepileptic drugs, new psychoactive drugs were confirmed in 102 cases, while no compound was detected in the other 3 cases. Estazolam, a benzodiazepine widely used in clinical practice as a sedative, hypnotic, and anti-anxiety drug, was the most frequently detected substance, occurring in 34.2% of the cases.
Most drugs in DBS could still be detected after storage for 3-5 years, but ambroxol, zopiclone, carbofuran, chlorpyrifos, and valproic acid were not detectable after 3-5 years of storage at room temperature. The components measured in DBS were consistent with those measured in whole blood at the collection time, thereby confirming that DBS samples have the advantage of stable storage at room temperature.
摘要：
目的：建立并验证了一种使用液相色谱-串联质谱（LC-MS/MS）的分析方法，用于筛选干血斑（DBSs）中的425种药物和毒物。
方法：将血液（20μL）点在WhatmanFTA™经典卡上以制备DBS样品，然后用150μL甲醇提取并使用多反应监测方法通过LC-MS/MS分析。
结果:化合物的检出限为0.1-10ng/mL。回收率和基质效应值分别为40.3-114.9%和40.2-118.4%，分别。该方法已成功应用于105名疑似药物中毒的人的DBS样品中，在室温下储存3-5年。三十三种药物,包括苯二氮卓类药物,抗精神病药,抗抑郁药,解热镇痛药，非甾体抗炎药，抗生素,抗癫痫药物,102例确诊了新的精神药物，而其他3例未检测到化合物。艾司唑仑,在临床实践中广泛用作镇静剂的苯二氮卓类药物，催眠,和抗焦虑药物，是最常见的检测物质，发生在34.2%的病例中。
结论：DBS中的大多数药物在储存3-5年后仍然可以检测到，但是氨溴索,佐匹克隆,克百威,毒死蜱,和丙戊酸在室温下储存3-5年后未检测到。在DBS中测量的成分与在采集时在全血中测量的成分一致。从而证实DBS样品具有在室温下稳定储存的优势。
方法：将血液（20μL）点在WhatmanFTA™经典卡上以制备DBS样品，
结果:化合物的检出限为0.1-10ng/mL。
结论：DBS中的大多数药物在储存3-5年后仍然可以检测到，
