PDF(Pubmed)
Abstract：
UNASSIGNED: This study aimed to determine whether controlled ovarian hyperstimulation (COH) parameters influence the incidence of de novo chromosomal abnormalities (> 4 Mb) in blastocysts and, thus, clinical pregnancy outcomes in preimplantation genetic testing (PGT).
UNASSIGNED: Couples who underwent preimplantation genetic testing for structural chromosome rearrangements (PGT-SR) and monogenic disorders (PGT-M) were included in this study. The relationships of maternal age, paternal age, stimulation protocol, exogenous gonadotropin dosage, duration of stimulation, number of oocytes retrieved and estradiol (E2) levels on human chorionic gonadotropin (hCG) trigger day with the incidence of de novo chromosomal abnormalities were assessed. Blastocysts were biopsied, and nuclear DNA was sequenced using next-generation sequencing (NGS). Clinical pregnancy outcomes after single euploid blastocyst transfers under different COH parameters were assessed.
UNASSIGNED: A total of 1,710 and 190 blastocysts were biopsied for PGT-SR and PGT-M, respectively. The rate of de novo chromosomal abnormalities was found to increase with maternal age (p< 0.001) and paternal age (p = 0.019) in the PGT-SR group. No significant differences in the incidence of de novo chromosomal abnormalities were seen for different maternal or paternal age groups between the PGT-SR and PGT-M groups (p > 0.05). Stratification analysis by gonadotropin dosage, stimulation protocol, duration of stimulation, number of retrieved oocytes and E2 levels on hCG trigger day revealed that de novo chromosomal abnormalities and clinical pregnancy outcomes were not correlated with COH parameters after adjusting for various confounding factors.
UNASSIGNED: The rate of de novo chromosomal abnormalities was found to increase with maternal or paternal age. COH parameters were found to not influence the incidence of de novo chromosomal abnormalities or clinical pregnancy outcomes.
UNASSIGNED: Couples who underwent preimplantation genetic testing for structural chromosome rearrangements (PGT-SR) and monogenic disorders (PGT-M) were included in this study. The relationships of maternal age, paternal age, stimulation protocol, exogenous gonadotropin dosage, duration of stimulation, number of oocytes retrieved and estradiol (E2) levels on human chorionic gonadotropin (hCG) trigger day with the incidence of de novo chromosomal abnormalities were assessed. Blastocysts were biopsied, and nuclear DNA was sequenced using next-generation sequencing (NGS). Clinical pregnancy outcomes after single euploid blastocyst transfers under different COH parameters were assessed.
UNASSIGNED: A total of 1,710 and 190 blastocysts were biopsied for PGT-SR and PGT-M, respectively. The rate of de novo chromosomal abnormalities was found to increase with maternal age (p< 0.001) and paternal age (p = 0.019) in the PGT-SR group. No significant differences in the incidence of de novo chromosomal abnormalities were seen for different maternal or paternal age groups between the PGT-SR and PGT-M groups (p > 0.05). Stratification analysis by gonadotropin dosage, stimulation protocol, duration of stimulation, number of retrieved oocytes and E2 levels on hCG trigger day revealed that de novo chromosomal abnormalities and clinical pregnancy outcomes were not correlated with COH parameters after adjusting for various confounding factors.
UNASSIGNED: The rate of de novo chromosomal abnormalities was found to increase with maternal or paternal age. COH parameters were found to not influence the incidence of de novo chromosomal abnormalities or clinical pregnancy outcomes.
摘要：
UNASSIGNED：本研究旨在确定控制性超促排卵（COH）参数是否会影响胚泡中新生染色体异常（>4Mb）的发生率，因此，植入前遗传学检测（PGT）的临床妊娠结局。
UNASSIGNED：本研究包括接受了结构染色体重排（PGT-SR）和单基因疾病（PGT-M）的植入前遗传学检测的夫妇。母亲年龄的关系，父系年龄,刺激方案，外源性促性腺激素剂量，刺激的持续时间，评估了人绒毛膜促性腺激素(hCG)触发日的卵母细胞数量和雌二醇(E2)水平以及从头染色体异常的发生率.对胚泡进行活检，使用下一代测序（NGS）对核DNA进行测序。评估不同COH参数下单个整倍体囊胚移植后的临床妊娠结局。
未经证实：共对1,710个和190个囊胚进行了PGT-SR和PGT-M的活检，分别。在PGT-SR组中，从头染色体异常的发生率随着母亲年龄（p<0.001）和父亲年龄（p=0.019）的增加而增加。PGT-SR组和PGT-M组之间，不同母亲或父亲年龄组的从头染色体异常发生率差异无统计学意义（p>0.05）。按促性腺激素剂量分层分析，刺激方案，刺激的持续时间，hCG触发日的卵母细胞数量和E2水平显示,在校正了各种混杂因素后,从头染色体异常和临床妊娠结局与COH参数无关.
UNASSIGNED：发现从头染色体异常的发生率随着母亲或父亲年龄的增加而增加。发现COH参数不影响从头染色体异常的发生率或临床妊娠结局。
UNASSIGNED：本研究包括接受了结构染色体重排（PGT-SR）和单基因疾病（PGT-M）的植入前遗传学检测的夫妇。母亲年龄的关系，父系年龄,刺激方案，外源性促性腺激素剂量，刺激的持续时间，评估了人绒毛膜促性腺激素(hCG)触发日的卵母细胞数量和雌二醇(E2)水平以及从头染色体异常的发生率.对胚泡进行活检，使用下一代测序（NGS）对核DNA进行测序。评估不同COH参数下单个整倍体囊胚移植后的临床妊娠结局。
未经证实：共对1,710个和190个囊胚进行了PGT-SR和PGT-M的活检，分别。在PGT-SR组中，从头染色体异常的发生率随着母亲年龄（p<0.001）和父亲年龄（p=0.019）的增加而增加。PGT-SR组和PGT-M组之间，不同母亲或父亲年龄组的从头染色体异常发生率差异无统计学意义（p>0.05）。按促性腺激素剂量分层分析，刺激方案，刺激的持续时间，hCG触发日的卵母细胞数量和E2水平显示,在校正了各种混杂因素后,从头染色体异常和临床妊娠结局与COH参数无关.
UNASSIGNED：发现从头染色体异常的发生率随着母亲或父亲年龄的增加而增加。发现COH参数不影响从头染色体异常的发生率或临床妊娠结局。
