Abstract：
Reactive oxygen species (ROS) are highly reactive chemical molecules containing oxygen. ROS play an important role in signaling and cell homeostasis at low and moderate concentrations. ROS could be a cause of damage to proteins, nucleic acids, lipids, membranes and organelles at high concentrations. There are a lot of cells that can produce ROS to maintain functional activity. It is known that metal nanoparticles can increase production of ROS in cells. However, the effect of cucurbiturils on ROS production is still unknown. In our study, we evaluated production of ROS by the immune (T-, B-lymphocytes, NK-cells) and non-immune cells (red blood cells, platelets), as well as tumor cells line (1301, K562) after treatment with cucurbiturils in vitro. Assessment of reactive oxide species (ROS) were provided by using dihydrorhodamine 123 (DHR 123). Fluorescence intensity and percentage DHR123 were measured by flow cytometry. Platelets, erythrocytes and activated T-helpers were changed the level of ROS production in response to stimulation with cucurbiturils. It was found that the percentage of these ROS-producing cells was reduced by cucurbiturils. Thus, cucurbiturils may affect the production of ROS by cells, but further research is needed in this area.
摘要：
活性氧（ROS）是含氧的高反应性化学分子。ROS在低和中等浓度下在信号传导和细胞稳态中起重要作用。ROS可能是蛋白质损伤的原因，核酸,脂质，高浓度的膜和细胞器。有很多细胞可以产生ROS来维持功能活性。已知金属纳米颗粒可以增加细胞中ROS的产生。然而,葫芦脲对ROS产生的影响尚不清楚。在我们的研究中,我们通过免疫评估了ROS的产生(T-，B淋巴细胞,NK细胞）和非免疫细胞（红细胞，血小板)，以及体外用葫芦脲处理后的肿瘤细胞系（1301，K562）。通过使用二氢罗丹明123(DHR123)提供反应性氧化物物质(ROS)的评估。荧光强度和百分比DHR123通过流式细胞术测量。血小板,响应于葫芦脲的刺激，红细胞和活化的T辅助细胞改变了ROS的产生水平。发现这些产生ROS的细胞的百分比被葫芦脲降低。因此，葫芦脲可能会影响细胞产生的ROS，但这方面还需要进一步的研究。
