Abstract：
Biodegradation is one of the safest and most economical methods for the elimination of toxic chlorophenols and crude oil from the environment. In this study, aerobic degradation of the aforementioned compounds by composite microbial agent B-Cl, which consisted of Bacillus B1 and B2 in a 3:2 ratio, was analyzed. The biodegradation mechanism of B-Cl was assessed based on whole genome sequencing, Fourier transform infrared spectroscopy and gas chromatographic analyses. B-Cl was most effective at reducing Cl- concentrations (65.17%) and crude oil biodegradation (59.18%) at 7 d, which was when the content of alkanes ≤ C30 showed the greatest decrease. Furthermore, adding B-Cl solution to soil significantly decreased the 2,4-DCP and oil content to below the detection limit and by 80.68%, respectively, and reconstructed of the soil microbial into a system containing more CPs-degrading (exaA, frmA, L-2-HAD, dehH, ALDH, catABE), aromatic compounds-degrading (pcaGH, catAE, benA-xylX, paaHF) and alkane- and fatty acid-degrading (alkB, atoB, fadANJ) microorganisms. Moreover, the presence of 2,4-DCP was the main hinder of the observed effects. This study demonstrates the importance of adding B-Cl solution to determine the interplay of CPs with microbes and accelerating oil degradation, which can be used for in-situ bioremediation of CPs and oil-contaminated soil.
摘要：
生物降解是从环境中消除有毒的氯酚和原油的最安全和最经济的方法之一。在这项研究中,复合微生物剂B-Cl对上述化合物的好氧降解，由芽孢杆菌B1和B2组成，比例为3:2，被分析。基于全基因组测序评估了B-Cl的生物降解机理，傅里叶变换红外光谱和气相色谱分析。B-Cl在7d时最有效地降低Cl-浓度（65.17％）和原油生物降解（59.18％），当烷烃含量≤C30时，下降幅度最大。此外，向土壤中添加B-Cl溶液显着降低了2,4-DCP和油含量，使其低于检测极限，降低了80.68％，分别,并将土壤微生物重建为含有更多CPs降解的系统(exaA，frmA,L-2-HAD,dehH,ALDH,catABE),芳香族化合物降解(pcaGH，catAE,benA-xylX,paaHF)和烷烃和脂肪酸降解(alkB，atoB,fadANJ)微生物。此外，2,4-DCP的存在是观察到的效果的主要障碍。这项研究证明了添加B-Cl溶液以确定CP与微生物的相互作用并加速油降解的重要性。可用于CPs和石油污染土壤的原位生物修复。
