Abstract：
Cell migration is a very dynamic process involving several chemical as well as biological interactions with other cells and the environment. Several models exist to study cell migration ranging from simple 2D in vitro cultures to more demanding 3D multicellular assays, to complex evaluation in animals. High-resolution 4D (XYZ, spatial + T, time dimension) intravital imaging using transgenic animals with a fluorescent label in cells of interest is a powerful tool to study cell migration in the correct environment. Here we describe an advanced dorsal skinfold chamber model to study endothelial cell and pericyte migration and association.
摘要：
细胞迁移是一个非常动态的过程，涉及与其他细胞和环境的几种化学和生物相互作用。存在几种模型来研究细胞迁移，从简单的2D体外培养到更苛刻的3D多细胞测定，对动物进行复杂的评估。高分辨率4D(XYZ,空间+T，时间维度）使用转基因动物在感兴趣的细胞中带有荧光标记的活体成像是研究正确环境中细胞迁移的有力工具。在这里，我们描述了一种先进的背侧皮褶室模型，以研究内皮细胞和周细胞的迁移和关联。
