Intraperitoneal injection of folinic acid, 5-fluorouracil, and OXA was administered on the SOS rat model for 7 weeks. The indexes of liver function were measured by biochemical kit. The ratio of liver weight to body weight was calculated. The pathological analysis of the liver was scored with the SOS scoring standard, fibrosis was evaluated with a four-point scale. The expression of inflammation factors was detected by Real-Time PCR, and the related indexes of IL-6/STAT3 were examined by Western blot analysis.
ILL down-regulated the portal vein pressure and alleviated the abnormal liver function of SOS rats and improved the liver lesions. ILL inhibited the SOS by inhibiting IL-6/STAT3.
ILL resistance to liver injury through inhibiting IL-6/STAT3 signal pathway.
方法:腹腔注射亚叶酸,5-氟尿嘧啶,在SOS大鼠模型上给予OXA7周。采用生化试剂盒测定肝功能指标。计算肝脏重量与体重的比率。肝脏病理分析采用SOS评分标准,纤维化用四点量表进行评估。实时荧光定量PCR检测炎症因子的表达,采用Westernblot分析检测IL-6/STAT3相关指标。
结果:ILL下调门静脉压力,减轻SOS大鼠肝功能异常,改善肝脏病变。ILL通过抑制IL-6/STAT3抑制SOS。
结论:ILL通过抑制IL-6/STAT3信号通路抵抗肝损伤。