Abstract：
Oxaliplatin (OXA) is easy to cause sinusoidal obstruction syndrome (SOS), leading to liver injury. Isolinderalactone (ILL), one of the main components of Lindera aggregate, has been reported to have a protecting effect on the liver. However, it is unclear whether ILL has a therapeutic effect on liver injury caused by OXA. This study aims to determine the effect of ILL on the prevention and treatment of OXA-induced liver injury and to provide a basis for the chemotherapy of gastrointestinal tumors.
Intraperitoneal injection of folinic acid, 5-fluorouracil, and OXA was administered on the SOS rat model for 7 weeks. The indexes of liver function were measured by biochemical kit. The ratio of liver weight to body weight was calculated. The pathological analysis of the liver was scored with the SOS scoring standard, fibrosis was evaluated with a four-point scale. The expression of inflammation factors was detected by Real-Time PCR, and the related indexes of IL-6/STAT3 were examined by Western blot analysis.
ILL down-regulated the portal vein pressure and alleviated the abnormal liver function of SOS rats and improved the liver lesions. ILL inhibited the SOS by inhibiting IL-6/STAT3.
ILL resistance to liver injury through inhibiting IL-6/STAT3 signal pathway.
Intraperitoneal injection of folinic acid, 5-fluorouracil, and OXA was administered on the SOS rat model for 7 weeks. The indexes of liver function were measured by biochemical kit. The ratio of liver weight to body weight was calculated. The pathological analysis of the liver was scored with the SOS scoring standard, fibrosis was evaluated with a four-point scale. The expression of inflammation factors was detected by Real-Time PCR, and the related indexes of IL-6/STAT3 were examined by Western blot analysis.
ILL down-regulated the portal vein pressure and alleviated the abnormal liver function of SOS rats and improved the liver lesions. ILL inhibited the SOS by inhibiting IL-6/STAT3.
ILL resistance to liver injury through inhibiting IL-6/STAT3 signal pathway.
摘要：
背景：奥沙利铂（OXA）容易引起正弦阻塞综合征（SOS），导致肝损伤。Isolinderalactone(ILL),Lindera骨料的主要成分之一，据报道对肝脏有防腐作用。然而,目前尚不清楚ILL是否对OXA引起的肝损伤有治疗作用。本研究旨在确定ILL对OXA肝损伤的防治作用,为胃肠道肿瘤的化疗提供依据。
方法：腹腔注射亚叶酸，5-氟尿嘧啶,在SOS大鼠模型上给予OXA7周。采用生化试剂盒测定肝功能指标。计算肝脏重量与体重的比率。肝脏病理分析采用SOS评分标准,纤维化用四点量表进行评估。实时荧光定量PCR检测炎症因子的表达,采用Westernblot分析检测IL-6/STAT3相关指标。
结果：ILL下调门静脉压力，减轻SOS大鼠肝功能异常，改善肝脏病变。ILL通过抑制IL-6/STAT3抑制SOS。
结论：ILL通过抑制IL-6/STAT3信号通路抵抗肝损伤。
方法：腹腔注射亚叶酸，5-氟尿嘧啶,在SOS大鼠模型上给予OXA7周。采用生化试剂盒测定肝功能指标。计算肝脏重量与体重的比率。肝脏病理分析采用SOS评分标准,纤维化用四点量表进行评估。实时荧光定量PCR检测炎症因子的表达,采用Westernblot分析检测IL-6/STAT3相关指标。
结果：ILL下调门静脉压力，减轻SOS大鼠肝功能异常，改善肝脏病变。ILL通过抑制IL-6/STAT3抑制SOS。
结论：ILL通过抑制IL-6/STAT3信号通路抵抗肝损伤。
