PDF(Pubmed)
Abstract：
The CaV1.1 voltage-gated Ca2+ channel carries L-type Ca2+ current and is the voltage-sensor for excitation-contraction (EC) coupling in skeletal muscle. Significant breakthroughs in the EC coupling field have often been close on the heels of technological advancement. In particular, CaV1.1 was the first voltage-gated Ca2+ channel to be cloned, the first ion channel to have its gating current measured and the first ion channel to have an effectively null animal model. Though these innovations have provided invaluable information regarding how CaV1.1 detects changes in membrane potential and transmits intra- and inter-molecular signals which cause opening of the channel pore and support Ca2+ release from the sarcoplasmic reticulum remain elusive. Here, we review current perspectives on this topic including the recent application of functional site-directed fluorometry.
摘要：
CaV1.1电压门控Ca2通道携带L型Ca2电流，是骨骼肌中兴奋-收缩（EC）耦合的电压传感器。EC耦合领域的重大突破通常紧随技术进步。特别是,CaV1.1是第一个被克隆的电压门控Ca2+通道，第一离子通道测量其门控电流,并且第一离子通道具有有效无效的动物模型。尽管这些创新提供了有关CaV1.1如何检测膜电位变化并传输导致通道孔打开并支持从肌浆网释放Ca2的分子内和分子间信号的宝贵信息，但仍然难以捉摸。这里,我们回顾了当前有关该主题的观点，包括功能性定点荧光测定法的最新应用。
