用单分子荧光原位杂交和免疫组织化学方法对斑马鱼胚胎单细胞中的 RNA 分子进行计数。Using single-molecule fluorescence in situ hybridization and immunohistochemistry to count RNA molecules in single cells in zebrafish embryos.-医云文献数字医云科研云海量医学决策数据服务

Abstract：

Taming gene expression variability is critical for robust pattern formation during embryonic development. Here, we describe an optimized protocol for single-molecule fluorescence in situ hybridization and immunohistochemistry in zebrafish embryos. We detail how to count segmentation clock RNAs and calculate their variability among neighboring cells. This approach is easily adaptable to count RNA numbers of any gene and calculate transcriptional variability among neighboring cells in diverse biological settings. For complete details on the use and execution of this protocol, please refer to Keskin et al. (2018),1 Zinani et al. (2021),2 and Zinani et al. (2022).3.

摘要：

驯服基因表达变异性对于胚胎发育过程中的稳健模式形成至关重要。这里,我们描述了斑马鱼胚胎单分子荧光原位杂交和免疫组织化学的优化方案.我们详细介绍了如何计算分割时钟RNA并计算它们在相邻细胞之间的变异性。这种方法很容易适用于计数任何基因的RNA数量，并计算不同生物学环境中相邻细胞之间的转录变异性。有关此协议的使用和执行的完整详细信息，请参考Keskin等人。(2018),1Zinani等人。(2021),2和Zinani等人。（2022年）。