Abstract：
In this study, genetic diversity and structure of 474 cultivated and 19 wild lablab (Lablab purpureus) accessions. were determined using 15 nuclear and 6 chloroplast SSR markers. The overall gene diversity was relatively low (0.3441). Gene diversity in the wild accessions (0.6059) was about two-folds greater than that in the cultivated accessions. In the wild accessions, gene diversity was greatest in the southern Africa, followed by East Africa. In the cultivated accessions, gene diversity was highest in the eastern Africa. The results suggested that South Africa is the center of origin and East Africa is the center of domestication of lablab. Different cluster analyses showed that 2-seeded-pod cultivated accessions (ssp. uncinatus) were clustered with wild accessions and that 4-(6)-seeded-pod cultivated accessions (ssp. purpureus and bengalensis) were intermingled. UPGMA tree suggested that ssp. purpureus and bengalensis were domesticated from 4-seeded-pod wild accessions of southern Africa. Haplotype network analysis based on nuclear SSRs revealed two domestication routes; the ssp. uncinatus is domesticated from 2-seeded-pod wild lablab (wild spp. uncinatus) from East Africa (Ethiopia), while the ssp. purpureus and bengalensis are domesticated from 4-seeded-pod wild lablab from Central Africa (Rwanda). These results are useful for understanding domestication and revising classification of lablab.
摘要：
在这项研究中,474种栽培和19种野生实验室（Lablabpurpureus）种质的遗传多样性和结构。使用15个核和6个叶绿体SSR标记确定。总体基因多样性相对较低(0.3441)。野生种质（0.6059）的基因多样性约为栽培种质的两倍。在野生物种中，基因多样性在南部非洲最大，其次是东非。在种植的种质中，基因多样性在东非最高。结果表明，南非是实验室的起源中心，东非是实验室的驯化中心。不同的聚类分析表明，2种子豆荚栽培的种质（ssp。uncinatus）聚集了野生种质，而4-（6）种豆荚栽培的种质（ssp。purpureus和bengalensis）混合在一起。UPGMA树建议ssp。purpureus和bengalensis是从南部非洲的4种子豆荚野生种质中驯化的。基于核SSR的单倍型网络分析揭示了两条驯化途径；ssp。uncinatus是从2种种子的野生实验室（野生物种。uncinatus)来自东非(埃塞俄比亚)，而ssp。purpureus和bengalensis是从中非（卢旺达）的4种子豆荚野生实验室驯化的。这些结果对于理解实验室的驯化和修订分类很有用。
