Abstract:
Butternut (Juglans cinerea L.), a species listed as endangered, is currently undergoing rapid decline due to habitat loss and the introduction of Ophiognomonia clavigignenti-juglandacearum, a non-native pathogen causing butternut canker. The decline of butternut has led to the development of cryobiological methods for the ex-situ conservation of the species since viability is not maintained using conventional methods. In this study, we assess the survivability and growth of butternut embryogenic axes into plantlets after 7 years of cryopreservation. Results show that cryopreservation is a highly effective method for the long-term storage of embryogenic axes since both survival and subsequent acclimatization into plantlets was successful with an average of 76% survival and 71% acclimatization, respectively. These results surpass the actual duration viability for non-cryogenic storage of butternut seed and we hope that future testing will result in longer term success of this cryobiological method and provide much needed germplasm for future restoration.
摘要:
Butternut(JuglanscinereaL.),被列为濒危物种,由于栖息地的丧失和虎尾草的引入,目前正在迅速下降,一种引起胡桃溃疡的非天然病原体。胡桃的减少导致了低温生物学方法的发展,用于该物种的非原位保护,因为使用常规方法无法维持生存能力。在这项研究中,经过7年的冷冻保存,我们评估了胡桃胚发生轴在幼苗中的生存能力和生长。结果表明,冷冻保存是长期保存胚轴的一种非常有效的方法,因为存活和随后的驯化均成功地成功进入小植株,平均存活率为76%,适应度为71%。分别。这些结果超过了胡桃种子非低温储存的实际持续时间,我们希望未来的测试将导致这种低温生物学方法的长期成功,并为未来的恢复提供急需的种质。