Abstract：
Butternut (Juglans cinerea L.), a species listed as endangered, is currently undergoing rapid decline due to habitat loss and the introduction of Ophiognomonia clavigignenti-juglandacearum, a non-native pathogen causing butternut canker. The decline of butternut has led to the development of cryobiological methods for the ex-situ conservation of the species since viability is not maintained using conventional methods. In this study, we assess the survivability and growth of butternut embryogenic axes into plantlets after 7 years of cryopreservation. Results show that cryopreservation is a highly effective method for the long-term storage of embryogenic axes since both survival and subsequent acclimatization into plantlets was successful with an average of 76% survival and 71% acclimatization, respectively. These results surpass the actual duration viability for non-cryogenic storage of butternut seed and we hope that future testing will result in longer term success of this cryobiological method and provide much needed germplasm for future restoration.
摘要：
Butternut(JuglanscinereaL.)，被列为濒危物种，由于栖息地的丧失和虎尾草的引入，目前正在迅速下降，一种引起胡桃溃疡的非天然病原体。胡桃的减少导致了低温生物学方法的发展，用于该物种的非原位保护，因为使用常规方法无法维持生存能力。在这项研究中,经过7年的冷冻保存，我们评估了胡桃胚发生轴在幼苗中的生存能力和生长。结果表明，冷冻保存是长期保存胚轴的一种非常有效的方法，因为存活和随后的驯化均成功地成功进入小植株，平均存活率为76％，适应度为71％。分别。这些结果超过了胡桃种子非低温储存的实际持续时间，我们希望未来的测试将导致这种低温生物学方法的长期成功，并为未来的恢复提供急需的种质。
