PDF(Pubmed)
Abstract：
Laboratory and clinical studies have revealed the importance of gut microbiota in children with severe pediatric pathological conditions such as severe acute malnutrition (SAM); however, under relatively milder conditions such as, failure to thrive (FTT), the role of the gut microbiota remains poorly characterized. Here, we analyzed stool samples from 54 subjects with a clinical diagnosis of failure to thrive (FTT), 49 preterm subjects with corrected normal growth (NFTT-pre), and 49 healthy subjects (NFTT) between 3-12 months of age using 16S rRNA gene sequencing. We observed that the clinical condition of FTT, age, head circumference, intrauterine growth restriction (IUGR), and feeding methods significantly affected gut microbiota. The microbiota age of subjects was significantly correlated with their anthropomorphic features, and the FTT subjects exhibited underdeveloped gut microbiota characterized by a significantly decreased microbiota-for-age Z-score (MAZ). The FTT and NFTT-pre groups exhibited an obvious disrupted developmental trajectory of gut microbiota across age, and the development of their alpha diversities and the observed OTU and Shannon indices were inadequate, particularly in subjects with FTT. Moreover, sequential colonization and enrichment of bacteria such as Bacteroides, Bifidobacterium, Streptococcus and most age-discriminatory bacterial taxa and their microbial functions were disorganized in FTT compared to that in NFTT. Our results revealed an underdevelopment of the gut microbiota in infants with failure to thrive that possesses potential clinical and practical importance.
摘要：
实验室和临床研究揭示了肠道菌群在患有严重儿科病理状况（如严重急性营养不良（SAM））的儿童中的重要性；然而，在相对温和的条件下，例如，未能茁壮成长(FTT)，肠道微生物群的作用仍未得到充分表征。这里,我们分析了54名临床诊断为茁壮成长失败（FTT）的受试者的粪便样本，49名生长正常的早产儿（NFTT-pre），和49名3-12月龄的健康受试者（NFTT）使用16SrRNA基因测序。我们观察到FTT的临床状况，年龄,头围,宫内生长受限（IUGR），和喂养方法显著影响肠道微生物群。受试者的微生物年龄与其拟人化特征显著相关，并且FTT受试者表现出不发达的肠道微生物群，其特征在于年龄的微生物群Z得分（MAZ）显着降低。FTT和NFTT-pre组表现出明显的肠道微生物群的发育轨迹随年龄的变化，它们的α多样性和观察到的OTU和Shannon指数的发展不足，特别是在FTT科目。此外，细菌如拟杆菌的顺序定植和富集，双歧杆菌,与NFTT相比，FTT中的链球菌和大多数年龄歧视细菌分类群及其微生物功能紊乱。我们的结果表明，未能茁壮成长的婴儿肠道微生物群发育不足，具有潜在的临床和实践重要性。
