Abstract：
Impaired wound healing is a severe complication of sufferers, related to prolonged wound closure, a high infection rate, and eventually disabilities of organs. To aid resolve this issue, we developed the electrospun polyvinyl alcohol and chitosan (PVA/CS) nanofibrous scaffold-loaded flaxseed extract. The scaffold containing 10 wt% of the extract indicated a three-dimensional cross-network with a nano-scale diameter (257 ± 37 nm) and smooth surface. Also, the relevant analyses confirmed high water absorption, porosity, and wettability of the scaffold. Fourier-transform infrared (FTIR), degradation, and mechanical studies revealed the intact presence and loading of the extract into the scaffold, the complete degradation over 48 h, and a high tensile elastic modulus. Besides, the advanced scaffold displayed remarkable anti-oxidant and could inhibit the growth of both Gram-positive and negative bacteria compared to the free PVA/CS scaffold. Desired fibroblast viability and blood compatibility of flaxseed-loaded scaffold endorsed the biocompatibility for wound zones. The in vitro studies showed that the flaxseed-loaded scaffold resulted in an accelerated wound healing process and 100 % closure of the scratched area within 48 h. The results obtained reveal that the flaxseed-loaded PVA/CS electrospun scaffold could be effectively applied for wound healing promotion.
摘要：
伤口愈合受损是患者的严重并发症，与长时间的伤口闭合有关，高感染率，最终导致器官残疾。为了帮助解决这个问题，我们开发了静电纺丝聚乙烯醇和壳聚糖（PVA/CS）纳米纤维支架负载亚麻籽提取物。含有10重量%提取物的支架显示出具有纳米级直径(257±37nm)和光滑表面的三维交叉网络。此外，相关分析证实了高吸水性，孔隙度,和支架的润湿性。傅里叶变换红外(FTIR),降解，机械研究揭示了提取物的完整存在和加载到支架中，在48小时内完全降解，和高拉伸弹性模量。此外,与游离的PVA/CS支架相比，高级支架显示出明显的抗氧化剂，并且可以抑制革兰氏阳性和阴性细菌的生长。亚麻籽负载支架的期望的成纤维细胞活力和血液相容性认可了伤口区的生物相容性。体外研究表明，亚麻籽负载的支架可在48h内加速伤口愈合过程，并使划痕区域100％闭合。获得的结果表明，亚麻籽负载的PVA/CS电纺支架可有效用于促进伤口愈合。
