PDF(Pubmed)
Abstract：
UNASSIGNED: Photodynamic antimicrobial therapy (PDAT) has been extensively studied because of its potential applications such as precise controllability, high spatiotemporal accuracy, and non-invasiveness. More importantly, it is difficult for bacteria to develop resistance to the aforementioned PDATs. However, the selectivity of traditional PDAT methods to bacteria is generally poor, so it has been proposed to introduce positively charged components such as quaternary ammonium salts to enhance the targeting of bacteria; however, they always possess high toxicity to normal cells. As a result, measures should be taken to enhance the targeting of bacteria and avoid side effects on normal cells.
UNASSIGNED: In our work, we creatively design a nanoplatform with high anti-bacterial efficiency, low side effects and its size is approximately 121 nm. BSA, as a nanocarrier, encapsulates the photosensitizer (E)-4-(4-(diphenylamino)styryl)-1-methylpyridin-1-ium with AIE properties named as BSA-Tpy, which increases its circulation time in vivo and improves the biocompatibility. Under acidic conditions (pH = 5.0), the surface positive charge of the BSA-Tpy is increased to +18.8 mV due to protonation of amine residues to achieve the targeting effect on bacteria. Besides, under the irradiation of white light, the BSA-Tpy will produce ROS to kill bacteria efficiently about 99.99% for both Gram-positive and Gram-negative bacteria, which shows the potential application value for the treatment of infected wounds.
UNASSIGNED: We have developed a feasible method for photodynamic antibacterial therapy, possessing excellent biocompatibility and high antibacterial efficiency with good fluorescence imaging property.
UNASSIGNED: In our work, we creatively design a nanoplatform with high anti-bacterial efficiency, low side effects and its size is approximately 121 nm. BSA, as a nanocarrier, encapsulates the photosensitizer (E)-4-(4-(diphenylamino)styryl)-1-methylpyridin-1-ium with AIE properties named as BSA-Tpy, which increases its circulation time in vivo and improves the biocompatibility. Under acidic conditions (pH = 5.0), the surface positive charge of the BSA-Tpy is increased to +18.8 mV due to protonation of amine residues to achieve the targeting effect on bacteria. Besides, under the irradiation of white light, the BSA-Tpy will produce ROS to kill bacteria efficiently about 99.99% for both Gram-positive and Gram-negative bacteria, which shows the potential application value for the treatment of infected wounds.
UNASSIGNED: We have developed a feasible method for photodynamic antibacterial therapy, possessing excellent biocompatibility and high antibacterial efficiency with good fluorescence imaging property.
摘要：
UNASSIGNED：光动力抗菌疗法（PDAT）由于其潜在的应用，例如精确的可控性而得到了广泛的研究，高时空精度，和非侵入性。更重要的是,细菌难以产生对上述PDAT的抗性。然而,传统PDAT方法对细菌的选择性普遍较差，因此，有人提出引入带正电荷的成分，如季铵盐，以增强细菌的靶向性；然而，它们总是对正常细胞具有高毒性。因此,应采取措施增强细菌的靶向性，避免对正常细胞的副作用。
未经评估：在我们的工作中，我们创造性地设计了一个抗菌效率高的纳米平台，低副作用，其大小约为121nm。BSA,作为纳米载体，封装光敏剂（E）-4-（4-（二苯基氨基）苯乙烯基）-1-甲基吡啶-1-um，具有AIE特性，命名为BSA-Tpy，这增加了其在体内的循环时间并改善了生物相容性。在酸性条件下（pH=5.0），由于胺残基的质子化，BSA-Tpy的表面正电荷增加到+18.8mV，以实现对细菌的靶向作用。此外,在白光的照射下，BSA-Tpy将产生ROS，以有效杀死革兰氏阳性和革兰氏阴性细菌约99.99％的细菌，对感染创面的治疗具有潜在的应用价值。
UNASSIGNED：我们已经开发了一种用于光动力抗菌疗法的可行方法，具有优异的生物相容性和高抗菌效率，具有良好的荧光成像性能。
未经评估：在我们的工作中，我们创造性地设计了一个抗菌效率高的纳米平台，低副作用，其大小约为121nm。BSA,作为纳米载体，封装光敏剂（E）-4-（4-（二苯基氨基）苯乙烯基）-1-甲基吡啶-1-um，具有AIE特性，命名为BSA-Tpy，这增加了其在体内的循环时间并改善了生物相容性。在酸性条件下（pH=5.0），由于胺残基的质子化，BSA-Tpy的表面正电荷增加到+18.8mV，以实现对细菌的靶向作用。此外,在白光的照射下，BSA-Tpy将产生ROS，以有效杀死革兰氏阳性和革兰氏阴性细菌约99.99％的细菌，对感染创面的治疗具有潜在的应用价值。
UNASSIGNED：我们已经开发了一种用于光动力抗菌疗法的可行方法，具有优异的生物相容性和高抗菌效率，具有良好的荧光成像性能。
