PDF(Pubmed)
Abstract：
Digital dermatitis (DD) associated with the presence of multiple Treponema spp. was recently described for the first time in European bison (Bison bonasus). DD is characterized by skin inflammation in the distal foot area in various ungulates. The objective of this proof of concept study was to test a treatment protocol adopted from cattle for its applicability in this wildlife species using five animals. Keratolytic salicylic acid paste was administered topically under bandages for seven days to enable removal of the affected skin. All interventions were performed under general anesthesia. To evaluate the treatment efficacy, photographs and biopsies were taken pre- and post-treatment. The biopsies were examined histologically, by PCR for the presence of different bacterial species, by Treponema-specific fluorescent in situ hybridization (FISH), and by transmission electron microscopy. Based on photographs, complete clinical healing of the 15 feet with macroscopical DD lesions was achieved. Histological examination showed mild to moderate dermatitis in 17/20 feet before, and in 12/20 feet after treatment. 17/20 feet were Treponema spp. PCR positive before, and none was positive after treatment. Dichelobacter nodosus, Fusobacterium necrophorum, and Porphyromonas levii could not be detected in any of the samples. By FISH and electron microscopy, Treponema spp. could be visualized in the stratum corneum before, but not after treatment. These results suggest that this treatment method can be applied as standard practice prior to transporting DD affected European bison to prevent the spread of this contagious disease.
摘要：
数字皮炎（DD）与多种密螺旋体的存在有关。最近在欧洲野牛（Bisonbonasus）中首次被描述。DD的特征在于各种有蹄类动物中远端足部区域的皮肤炎症。此概念验证研究的目的是使用五种动物测试牛采用的治疗方案在该野生动植物物种中的适用性。在绷带下局部施用角质层水解水杨酸糊剂七天以使得能够去除受影响的皮肤。所有干预措施均在全身麻醉下进行。为了评估治疗效果，在治疗前后拍摄照片和活检.活检进行了组织学检查，通过PCR检测不同细菌种类的存在，通过螺旋体特异性荧光原位杂交（FISH），和透射电子显微镜。根据照片,15英尺宏观DD病变的临床完全愈合。组织学检查显示17/20英尺前轻度至中度皮炎，治疗后12/20英尺。17/20英尺是密螺旋体属。PCR阳性前，治疗后无阳性。结节性双螺旋杆菌,坏死梭杆菌,并且在任何样品中都无法检测到卟啉。通过FISH和电子显微镜，密螺旋体属。可以在角质层之前可视化，但不是在治疗后。这些结果表明，在运输受DD影响的欧洲野牛之前，可以将这种治疗方法用作标准做法，以防止这种传染性疾病的传播。
