Abstract：
CTP synthase (CTPS) forms a filamentous structure termed the cytoophidium in all three domains of life. The female reproductive system of Drosophila is an excellent model for studying the physiological function of cytoophidia. Here, we use CTPSH355A, a point mutation that destroys the cytoophidium-forming ability of CTPS, to explore the in vivo function of cytoophidia. In CTPSH355A egg chambers, we observe the ingression and increased heterogeneity of follicle cells. In addition, we find that the cytoophidium-forming ability of CTPS, rather than the protein level, is the cause of the defects observed in CTPSH355A mutants. To sum up, our data indicate that cytoophidia play an important role in maintaining the integrity of follicle epithelium.
摘要：
CTP合酶(CTPS)在生命的所有三个结构域中形成称为胞质的丝状结构。果蝇的雌性生殖系统是研究胞嘧啶生理功能的极好模型。这里,我们使用CTPSH355A，点突变会破坏CTPS的细胞形成能力，探讨胞嘧啶在体内的功能。在CTPSH355A卵室中，我们观察到卵泡细胞的侵入和异质性增加。此外,我们发现CTPS的细胞形成能力，而不是蛋白质水平，是CTPSH355A突变体中观察到的缺陷的原因。总而言之,我们的数据表明，胞嘧啶在维持卵泡上皮的完整性中起着重要作用。
