PDF(Pubmed)
Abstract：
Pseudorabies (PR) is an important infectious disease of swine that causes enormous economic losses to the swine industry in China. Immunization with vaccines is a routine practice to control this disease. PRV inactivated vaccines usually require a booster vaccination to provide complete immune protection. Therefore, Astragalus saponins (AST) have been added as an immunopotentiator to improve the immune efficacy and reduce the immunization times for the PRV inactivated vaccine. The results in mice have shown that a single dose of AST-adjuvanted PRV inactivated vaccine promoted higher production of gB-specific IgG, IgG1, and IgG2a and neutralizing antibody, secretion of Th1-type (IFN-γ) and Th2-type (IL-4) cytokines, and lymphocyte proliferation than mice immunized without AST. Compared to mice immunized without AST, a single dose of the AST-adjuvanted PRV inactivated vaccine improved the survival percentage of mice and reduced the PRV viral loads in the lungs and brains after lethal challenge. In summary, AST was an effective immunopotentiator to improve the immune efficacy of a single dose PRV inactivated vaccine.
摘要：
伪狂犬病是一种重要的猪传染病,给我国养猪业造成了巨大的经济损失。用疫苗免疫是控制这种疾病的常规做法。PRV灭活疫苗通常需要加强疫苗接种以提供完全的免疫保护。因此,已添加黄芪皂苷（AST）作为免疫增强剂，以提高PRV灭活疫苗的免疫效力并减少免疫次数。在小鼠中的结果表明，单剂量的AST佐剂PRV灭活疫苗可促进gB特异性IgG的产生。IgG1和IgG2a和中和抗体，Th1型（IFN-γ）和Th2型（IL-4）细胞因子的分泌，和淋巴细胞增殖比没有AST免疫的小鼠。与无AST免疫的小鼠相比，单剂量的AST佐剂PRV灭活疫苗可提高小鼠的存活率,并降低致命性攻击后肺和大脑中的PRV病毒载量.总之,AST是提高单剂量PRV灭活疫苗免疫效力的有效免疫增强剂。
