PDF(Pubmed)
Abstract：
The spread of pathogenic bacteria in coastal waters endangers the health of the local people and jeopardizes the safety of the marine environment. However, their dynamics during seasonal hypoxia in the Bohai Sea (BHS) have not been studied. Here, pathogenic bacteria were detected from the 16S rRNA gene sequencing database and were used to explore their dynamics and driving factors with the progressively deoxygenating in the BHS. Our results showed that pathogenic bacteria were detected in all samples, accounting for 0.13 to 24.65% of the total number of prokaryotic sequences in each sample. Pathogenic Proteobacteria was dominated in all samples, followed by Firmicutes, Actinobacteria, Tenericutes, and Bacteroidetes, etc. β-diversity analysis showed that pathogenic bacteria are highly temporally heterogeneous and regulated by environmental factors. According to RDA analysis, these variations may be influenced by salinity, ammonia, DO, phosphate, silicate, and Chl a. Additionally, pathogenic bacteria in surface water and hypoxia zone were found to be significantly separated in August. The vertical distribution of pathogenic bacterial communities is influenced by several variables, including DO and nutrition. It is noteworthy that the hypoxia zones increase the abundance of certain pathogenic genera, especially Vibrio and Arcobacter, and the stability of the pathogenic bacterial community increased from May to August. These phenomena indicate that the central Bohai Sea is threatened by an increasingly serious pathogenic community from May to August. And the developing hypoxia zone in the future may intensify this phenomenon and pose a more serious threat to human health. This study provides new insight into the changes of pathogenic bacteria in aquatic ecosystems and may help to make effective policies to control the spread of pathogenic bacteria.
摘要：
致病菌在近岸海域的传播危害当地人民的健康，危害海洋环境安全。然而,尚未研究它们在渤海（BHS）季节性缺氧期间的动态。这里,从16SrRNA基因测序数据库中检测到病原菌，并用于探索其动力学和驱动因素随着BHS中逐渐脱氧。我们的结果表明，在所有样本中都检测到致病菌，占每个样本中原核序列总数的0.13～24.65%。在所有样本中，病原性变形杆菌占主导地位，其次是Firmicutes，放线菌,Tenericutes,和拟杆菌,等。β多样性分析表明，病原菌具有高度的时间异质性，并受环境因素的调节。根据RDA分析,这些变化可能受到盐度的影响，氨,DO,磷酸盐,硅酸盐,和Chla。此外，8月发现地表水和缺氧区的病原菌明显分离。病原菌群落的垂直分布受几个变量的影响，包括DO和营养。值得注意的是，缺氧区增加了某些病原属的丰度，尤其是弧菌和杆菌,5月至8月病原菌群落稳定性增强。这些现象表明，从5月到8月，渤海中部受到日益严重的病原群落的威胁。未来发展中的缺氧区可能会加剧这种现象，对人类健康构成更严重的威胁。该研究为水生生态系统中病原菌的变化提供了新的见解，可能有助于制定有效的策略来控制病原菌的传播。
