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Abstract：
UNASSIGNED: Bone marrow mesenchymal stem cells (BMSCs) are a promising cell type for tissue engineering, however, the application of BMSCs is largely hampered by the limited number harvested from bone marrow cells. The methods or strategies that focused on promoting the capacity of BMSCs expansion ex vivo become more and more important. Tanshinone IIA (Tan IIA), the main active components of Danshen, has been found to promote BMSCs proliferation, but the underlying mechanism is still unclear. The aim of this study is to explore the effect and underlying mechanism of Tan IIA on the expansion capacity of hBMSCs ex vivo.
UNASSIGNED: In this present study, the effect of Tan IIA on the expansion capacity of BMSCs from human was investigated, and quantitative proteome analysis was applied furtherly to identify the differentially expressed proteins (DEPs) and the molecular signaling pathways in Tan IIA-treated hBMSCs. Finally, molecular biology skills were employed to verify the proposed mechanism of Tan IIA in promoting hBMSCs expansion.
UNASSIGNED: The results showed that a total of 84 DEPs were identified, of which 51 proteins were upregulated and 33 proteins were downregulated. Besides, Tan IIA could promote hBMSCs proliferation by regulating the progression of S phase via increasing the release of fibroblast growth factor 2 (FGF2), FGF-mediated PI3K/AKT signaling pathways may play an important role in Tan IIA\'s effect on hBMSCs expansion.
UNASSIGNED: This study employed molecular biology skills combined with quantitative proteome analysis, to some extent, clarified the mechanism of Tan IIA\'s effect on promoting hBMSCs proliferation, and will give a hint that Tan IIA may have the potential to be used for BMSCs applications in cell therapies in the future.
UNASSIGNED: In this present study, the effect of Tan IIA on the expansion capacity of BMSCs from human was investigated, and quantitative proteome analysis was applied furtherly to identify the differentially expressed proteins (DEPs) and the molecular signaling pathways in Tan IIA-treated hBMSCs. Finally, molecular biology skills were employed to verify the proposed mechanism of Tan IIA in promoting hBMSCs expansion.
UNASSIGNED: The results showed that a total of 84 DEPs were identified, of which 51 proteins were upregulated and 33 proteins were downregulated. Besides, Tan IIA could promote hBMSCs proliferation by regulating the progression of S phase via increasing the release of fibroblast growth factor 2 (FGF2), FGF-mediated PI3K/AKT signaling pathways may play an important role in Tan IIA\'s effect on hBMSCs expansion.
UNASSIGNED: This study employed molecular biology skills combined with quantitative proteome analysis, to some extent, clarified the mechanism of Tan IIA\'s effect on promoting hBMSCs proliferation, and will give a hint that Tan IIA may have the potential to be used for BMSCs applications in cell therapies in the future.
摘要：
未经授权：骨髓间充质干细胞（BMSCs）是一种有前途的组织工程细胞类型，然而,BMSCs的应用在很大程度上受到骨髓细胞收获数量有限的阻碍。专注于促进BMSCs离体扩增能力的方法或策略变得越来越重要。丹参酮IIA(TanIIA),丹参的主要活性成分，已发现促进BMSCs增殖，但潜在的机制仍不清楚。本研究旨在探讨TanIIA对hBMSCs体外扩增能力的影响及潜在机制。
未经批准：在本研究中，研究了TanIIA对人骨髓间充质干细胞扩增能力的影响,和定量蛋白质组分析进一步应用于鉴定TanIIA处理的hBMSCs中的差异表达蛋白（DEPs）和分子信号通路。最后,采用分子生物学技术验证了TanIIA促进hBMSCs扩增的机制。
UNASSIGNED：结果表明，总共确定了84个DEP，其中51种蛋白质上调，33种蛋白质下调。此外,TanIIA可以通过增加成纤维细胞生长因子2(FGF2)的释放来调节S期进程,从而促进hBMSCs的增殖,FGF介导的PI3K/AKT信号通路可能在TanIIA对hBMSCs扩增的影响中起重要作用。
UNASSIGNED：本研究采用分子生物学技能结合定量蛋白质组分析，在某种程度上,阐明了TanIIA促进hBMSCs增殖的作用机制,并暗示TanIIA未来可能有潜力用于BMSCs在细胞治疗中的应用。
未经批准：在本研究中，研究了TanIIA对人骨髓间充质干细胞扩增能力的影响,和定量蛋白质组分析进一步应用于鉴定TanIIA处理的hBMSCs中的差异表达蛋白（DEPs）和分子信号通路。最后,采用分子生物学技术验证了TanIIA促进hBMSCs扩增的机制。
UNASSIGNED：结果表明，总共确定了84个DEP，其中51种蛋白质上调，33种蛋白质下调。此外,TanIIA可以通过增加成纤维细胞生长因子2(FGF2)的释放来调节S期进程,从而促进hBMSCs的增殖,FGF介导的PI3K/AKT信号通路可能在TanIIA对hBMSCs扩增的影响中起重要作用。
UNASSIGNED：本研究采用分子生物学技能结合定量蛋白质组分析，在某种程度上,阐明了TanIIA促进hBMSCs增殖的作用机制,并暗示TanIIA未来可能有潜力用于BMSCs在细胞治疗中的应用。
