Abstract：
Rheum palmatum, a well-known Traditional Chinese Medicines (TCM), has been used for medical purposes for thousand years in China. However, endophyte diversity of R. palmatum among different tissues and ages is still not revealed. In this study, we used 16S and ITS amplicon sequencing and combined with PICRUSt and FUNGuild to compare endophyte diversity and ecological function among different tissues and ages of R. palmatum. The results showed that the diversity and OTUs (Operational taxonomic units) abundance of endophytic fungi and bacteria of R. palmatum differed among different tissues and ages. The predictive function analysis showed that metabolism was main function of endophytic bacteria in different tissue and year samples, while saprotroph was dominant trophic mode of endophytic fungi in different year samples. The dominant trophic modes of endophytic fungi were saprotroph, pathotroph-symbiotroph and symbiotroph, and relative abundances differed in the different tissue samples. Our results elucidated the comprehensive diversity and composition profiles of endophytes in different tissues and year of R. palmatum. Our data offered pivotal information to clarify the role of endophytes in the production of R. palmatum and its important metabolites.
摘要：
掌叶大黄,一种著名的中药（TCM），在中国已经被用于医疗目的一千年了。然而,不同组织和年龄之间的掌叶草内生菌多样性仍未揭示。在这项研究中,我们使用16S和ITS扩增子测序，并与PICRUSt和FUNGuild结合使用，比较了不同组织和年龄的R.palmatum的内生菌多样性和生态功能。结果表明，不同组织和年龄的掌叶树内生真菌和细菌的多样性和OTU（操作分类单位）丰度不同。预测功能分析显示,代谢是内生细菌在不同组织和年份样本中的主要功能,腐生菌是不同年份内生真菌的主要营养模式。内生真菌的优势营养模式是腐生，病菌-共生菌和共生菌，不同组织样本的相对丰度不同。我们的研究结果阐明了掌叶树不同组织和年份内生菌的综合多样性和组成谱。我们的数据提供了关键信息，以阐明内生菌在R.palmatum及其重要代谢产物的生产中的作用。
