Abstract:
Plant research and breeding depends on plant genotypes; therefore, genotype authenticity of accessions is the basic requirement for users of gene banks. Surprisingly, this extremely important topic is rarely reported in the scientific community. Non-authentic are accessions that are mislabelled and undesirable genotypes of heterogeneous accessions. In barley, we try to uncover both named problems on the basis of postulated major powdery mildew resistance genes. These are diverse, environmentally stable and their use is well documented and suitable for genotype characterization. In this contribution, we postulate resistance genes in 15 varieties represented by 157 derived lines of 32 accessions originating from seven foreign gene banks and compare these findings with previous results including those 15 identically labelled varieties from our domestic gene bank. We found that 37.5% of the gene bank accessions investigated herein were heterogeneous, and at least 20.0% were mislabelled. A large-scale molecular characterisation of varieties is now being carried out, and using authentic varieties must be one of the key requirements. Therefore, accessions of each variety from a minimum of three gene banks whose identity has been verified by reliable methods should be compared before starting new experiments. These will involve molecular varietal characterisation to serve as a foundation for future plant science research and effective crop improvement.
摘要:
植物研究和育种取决于植物基因型;因此,种质的基因型真实性是基因库用户的基本要求。令人惊讶的是,这个极其重要的话题在科学界很少报道。非真实是错误标记的种质和异质种质的不良基因型。在大麦中,我们试图根据假定的主要白粉病抗性基因来揭示这两个命名问题。这些是多种多样的,环境稳定,它们的使用有据可查,适用于基因型表征。在这一贡献中,我们假设15个品种的抗性基因,这些品种由来自7个外国基因库的32个种质的157个衍生品系代表,并将这些发现与以前的结果进行比较,包括来自我们国内基因库的15个相同标记的品种。我们发现本文研究的基因库中有37.5%是异质的,和至少20.0%被错误标记。现在正在对品种进行大规模的分子表征,使用正宗品种必须是关键要求之一。因此,在开始新实验之前,应比较至少三个基因库中每个品种的种质,这些基因库的身份已通过可靠的方法进行验证。这些将涉及分子品种表征,以作为未来植物科学研究和有效作物改良的基础。