OBJECTIVE: This study aimed to investigate the behavioral effects of prolonged MS in the offspring of mice using the EPM and MB tests.
METHODS: Male BALB/c mice were isolated from their mothers for 4 h each day during the first 30 days after birth. On day 50 postnatal, groups of separated and non-separated mice (n = 18/each group) were subjected to the EPM and MB tests for comparative behavioral evaluations. In addition, the locomotor activity of mice was evaluated using the actophotometer test.
RESULTS: The findings of the EPM test revealed that separated mice exhibited anxiolytic-like behaviors, as evidenced by a significant increase in the latency to closed arms and the time spent in the open arms compared with non-separated mice. Separated mice also showed compulsive burying activity in the MB test, as determined by a significant increase in the number of buried marbles. The results of the actophotometer test did not show any significant change in locomotor activity.
CONCLUSIONS: Prolonged MS caused the adult offspring of mice to exhibit a decrease in anxiety state and increased compulsive burying activity, which were not associated with a change in locomotor activity. Further investigations with validated tests are needed to support these findings.
目的:本研究旨在使用EPM和MB测试研究长期MS对小鼠后代的行为影响。
方法:雄性BALB/c小鼠在出生后的前30天每天从其母亲中分离4小时。产后第50天,对分离的和非分离的小鼠组(n=18/每组)进行EPM和MB测试以进行比较行为评价。此外,小鼠的运动活动用手电光度计试验进行评价.
结果:EPM测试的结果显示,分离的小鼠表现出抗焦虑样行为,与未分开的小鼠相比,闭合臂的潜伏期和在开放臂中花费的时间显着增加。分开的小鼠在MB测试中也表现出强迫性掩埋活动,这取决于埋藏的大理石数量的显着增加。手电光度计测试的结果未显示运动活动的任何显着变化。
结论:延长MS导致小鼠的成年后代表现出焦虑状态的降低和强迫性埋葬活动的增加,与运动活动的变化无关。需要使用经过验证的测试进行进一步的调查以支持这些发现。