Abstract：
Ethical livestock production is currently a major concern for consumers. In parallel, research has shown that transport duration is an important factor affecting animal welfare and has a negative impact on the final product quality and on the production cost. This study applied proteomics methods to the animal stress/welfare problem in pigs muscle-exudate with the aim to identify proteins indicative of molecular processes underpinning transport stress and to better characterise this species as a biomedical model. A broader perspective of the problem was obtained by applying label-free LC-MS to characterise the proteome response to transport stress (short or long road transportation) in pigs within the same genetic line. A total of 1,464 proteins were identified, following statistical analysis 66 proteins clearly separating pigs subject to short road transportation and pigs subject long road transportation. These proteins were mainly involved in cellular and metabolic processes. Catalase and stress-induced phosphoprotein-1 were further confirmed by Western blot as being involved in the process of self-protection of the cells in response to stress. This study provide an insight into the molecular processes that are involved in pig adaptability to transport stress and are a step-forward for the development of an objective evaluation method of stress in order to improve animal care and management in farm animals.
摘要：
道德牲畜生产目前是消费者的主要关注点。并行,研究表明，运输持续时间是影响动物福利的重要因素，对最终产品质量和生产成本有负面影响。这项研究将蛋白质组学方法应用于猪肌肉渗出物中的动物应激/福利问题，目的是鉴定指示支撑运输应激的分子过程的蛋白质，并更好地将该物种表征为生物医学模型。通过应用无标记LC-MS来表征同一遗传系内猪对运输应激（短期或长期公路运输）的蛋白质组反应，可以获得更广泛的问题。总共鉴定了1464种蛋白质，经过统计分析，66种蛋白质清楚地将短途运输猪和长途运输猪分开。这些蛋白质主要参与细胞和代谢过程。通过Western印迹进一步证实过氧化氢酶和应激诱导的磷蛋白-1参与细胞响应应激的自我保护过程。这项研究提供了对猪适应运输应激的分子过程的见解，并且为开发客观的应激评估方法迈出了一步，以改善农场动物的动物护理和管理。
