PDF(Pubmed)
Abstract：
UNASSIGNED: Infective endocarditis (IE) is an uncommon disease with high morbidity and mortality rates, which often develops from oral bacterial species entering circulation.
UNASSIGNED: We compared oral microbiome profiles of three groups: IE patients (N 9 patients; n = 27 samples), disease controls at risk for IE (N = 28; n = 84), and healthy controls (N = 37; n = 111). Bacterial species in IE patients\' blood cultures were identified for comparison with matched oral samples.
UNASSIGNED: Oral microbiome profiles were obtained from buccal mucosa, saliva, and tongue samples for all three groups and from sub- and supra-gingival plaque samples of the IE group (N = 9; n = 16) and disease controls (N = 28; n = 54). Alpha- and beta-diversities were determined based on relative abundance data. Discriminative species were identified by LEfSe, post hoc Mann-Whitney, and ROC analyses. Identity of the bacterial species in IE patients\' blood cultures was confirmed by 16S-rRNA gene Sanger sequencing.
UNASSIGNED: Alpha- and beta-diversities differed between groups. Discriminative IE-associated species were identified, e.g. Haemophilus parainfluenzae and Streptococcus sanguinis. Two blood isolates were Staphylococcus aureus, also identified in one matched saliva sample. Streptococcus mutans was present in one patient\'s plaque samples and blood culture.
UNASSIGNED: Oral microbiomes of IE, non-IE disease controls, and healthy controls differed significantly. A better understanding of IE-related bacterial-host interactions is warranted.
UNASSIGNED: We compared oral microbiome profiles of three groups: IE patients (N 9 patients; n = 27 samples), disease controls at risk for IE (N = 28; n = 84), and healthy controls (N = 37; n = 111). Bacterial species in IE patients\' blood cultures were identified for comparison with matched oral samples.
UNASSIGNED: Oral microbiome profiles were obtained from buccal mucosa, saliva, and tongue samples for all three groups and from sub- and supra-gingival plaque samples of the IE group (N = 9; n = 16) and disease controls (N = 28; n = 54). Alpha- and beta-diversities were determined based on relative abundance data. Discriminative species were identified by LEfSe, post hoc Mann-Whitney, and ROC analyses. Identity of the bacterial species in IE patients\' blood cultures was confirmed by 16S-rRNA gene Sanger sequencing.
UNASSIGNED: Alpha- and beta-diversities differed between groups. Discriminative IE-associated species were identified, e.g. Haemophilus parainfluenzae and Streptococcus sanguinis. Two blood isolates were Staphylococcus aureus, also identified in one matched saliva sample. Streptococcus mutans was present in one patient\'s plaque samples and blood culture.
UNASSIGNED: Oral microbiomes of IE, non-IE disease controls, and healthy controls differed significantly. A better understanding of IE-related bacterial-host interactions is warranted.
摘要：
未经证实：感染性心内膜炎（IE）是一种罕见的疾病，具有很高的发病率和死亡率，通常从口腔细菌物种进入循环发展。
UNASSIGNED：我们比较了三组的口腔微生物组概况：IE患者（N9名患者；n=27个样本），有IE风险的疾病控制（N=28；n=84），和健康对照(N=37;n=111)。确定了IE患者血液培养物中的细菌种类，以与匹配的口腔样本进行比较。
未经证实：口腔微生物组谱是从口腔粘膜获得的，唾液,所有三组以及IE组（N=9；n=16）和疾病对照（N=28；n=54）的牙龈下和牙龈上菌斑样本。根据相对丰度数据确定α和β多样性。区分物种由LEfSe鉴定，事后Mann-Whitney,和ROC分析。通过16S-rRNA基因Sanger测序确认了IE患者血液培养物中细菌的种类。
UnASSIGNED：组之间的Alpha和beta多样性不同。确定了与IE相关的歧视性物种，例如副流感嗜血杆菌和血链球菌。两个血液分离物是金黄色葡萄球菌，也在一个匹配的唾液样本中鉴定。变形链球菌存在于一名患者的斑块样本和血培养物中。
未经批准：IE的口腔微生物组，非IE疾病控制，和健康控制显著不同。有必要更好地理解IE相关的细菌-宿主相互作用。
UNASSIGNED：我们比较了三组的口腔微生物组概况：IE患者（N9名患者；n=27个样本），有IE风险的疾病控制（N=28；n=84），和健康对照(N=37;n=111)。确定了IE患者血液培养物中的细菌种类，以与匹配的口腔样本进行比较。
未经证实：口腔微生物组谱是从口腔粘膜获得的，唾液,所有三组以及IE组（N=9；n=16）和疾病对照（N=28；n=54）的牙龈下和牙龈上菌斑样本。根据相对丰度数据确定α和β多样性。区分物种由LEfSe鉴定，事后Mann-Whitney,和ROC分析。通过16S-rRNA基因Sanger测序确认了IE患者血液培养物中细菌的种类。
UnASSIGNED：组之间的Alpha和beta多样性不同。确定了与IE相关的歧视性物种，例如副流感嗜血杆菌和血链球菌。两个血液分离物是金黄色葡萄球菌，也在一个匹配的唾液样本中鉴定。变形链球菌存在于一名患者的斑块样本和血培养物中。
未经批准：IE的口腔微生物组，非IE疾病控制，和健康控制显著不同。有必要更好地理解IE相关的细菌-宿主相互作用。
