PDF(Pubmed)
Abstract：
Early embryonic arrest (EEA) leads to repeated cessation of fresh cycles among infertile women undergoing in vitro fertilization (IVF). Whether the levels of some essential trace elements [copper (Cu), zinc (Zn), selenium (Se) and cobalt (Co)] in the bodies of women are related to the risk of EEA warrants study.
Our study aimed to investigate the associations of peripheral blood levels of Cu, Zn, Se, and Co and their mixtures with the risk of EEA.
A total of 74 EEA cases (123 IVF cycles) and 157 controls (180 IVF cycles) from the reproductive center of the First Affiliated Hospital of Anhui Medical University in Hefei, China, between June 2017 and March 2020 were included in our study. Demographic and clinical data were collected from electronic medical records. Cu, Zn, Se, and Co levels were measured in blood samples collected on the day of oocyte retrieval when infertile women entered clinical treatment for the first time using an inductively coupled plasma mass spectrometer (ICP-MS). Generalized estimating equation (GEE) models were used to evaluate the associations of four essential trace element concentrations individually with the risk of EEA, and Bayesian kernel machine regression (BKMR) was used to explore the associations between four essential trace element mixtures and the risk of EEA.
Se concentrations of infertile women were significantly lower in the case group compared with the control group. Co levels were significantly higher in the case group compared with the control group. The differences in Cu and Zn concentrations between the two groups were not significant. Based on single-metal models, Co was positively associated with the risk of EEA before and after adjustment for all confounders (odd ratio (OR) = 1.72, 95% confidence interval (CI): 1.18-2.52; OR = 2.27, 95% CI: 1.37-3.77, respectively), and Se was negatively associated with the risk of EEA before adjustment for all confounders (OR = 0.18, 95% CI: 0.07-0.51). BKMR analyses showed that Se was significantly and negatively associated with the risk of EEA when all the other three metals (Cu, Zn, and Co) were fixed at the 25th, 50th, or 75th percentiles, whereas Zn displayed a significant and positive association with the risk of EEA when all the other three metals (Cu, Se and Co) were fixed at the 25th, 50th, or 75th percentiles. Co did not show any effect on the risk of EEA when all the other metals (Cu, Zn, and Se) were fixed at the 25th, 50th, or 75th percentiles. In addition, an increasing trend of the joint effect of four essential trace elements on the risk of EEA was found, although it was not statistically significant.
The levels of essential trace elements (Cu, Zn, Se, and Co) might correlate with the risk of EEA to some extent. The present study might provide a real-world perspective on the relationship between essential trace elements and the risk of EEA when considering them as a single element or as mixtures.
Our study aimed to investigate the associations of peripheral blood levels of Cu, Zn, Se, and Co and their mixtures with the risk of EEA.
A total of 74 EEA cases (123 IVF cycles) and 157 controls (180 IVF cycles) from the reproductive center of the First Affiliated Hospital of Anhui Medical University in Hefei, China, between June 2017 and March 2020 were included in our study. Demographic and clinical data were collected from electronic medical records. Cu, Zn, Se, and Co levels were measured in blood samples collected on the day of oocyte retrieval when infertile women entered clinical treatment for the first time using an inductively coupled plasma mass spectrometer (ICP-MS). Generalized estimating equation (GEE) models were used to evaluate the associations of four essential trace element concentrations individually with the risk of EEA, and Bayesian kernel machine regression (BKMR) was used to explore the associations between four essential trace element mixtures and the risk of EEA.
Se concentrations of infertile women were significantly lower in the case group compared with the control group. Co levels were significantly higher in the case group compared with the control group. The differences in Cu and Zn concentrations between the two groups were not significant. Based on single-metal models, Co was positively associated with the risk of EEA before and after adjustment for all confounders (odd ratio (OR) = 1.72, 95% confidence interval (CI): 1.18-2.52; OR = 2.27, 95% CI: 1.37-3.77, respectively), and Se was negatively associated with the risk of EEA before adjustment for all confounders (OR = 0.18, 95% CI: 0.07-0.51). BKMR analyses showed that Se was significantly and negatively associated with the risk of EEA when all the other three metals (Cu, Zn, and Co) were fixed at the 25th, 50th, or 75th percentiles, whereas Zn displayed a significant and positive association with the risk of EEA when all the other three metals (Cu, Se and Co) were fixed at the 25th, 50th, or 75th percentiles. Co did not show any effect on the risk of EEA when all the other metals (Cu, Zn, and Se) were fixed at the 25th, 50th, or 75th percentiles. In addition, an increasing trend of the joint effect of four essential trace elements on the risk of EEA was found, although it was not statistically significant.
The levels of essential trace elements (Cu, Zn, Se, and Co) might correlate with the risk of EEA to some extent. The present study might provide a real-world perspective on the relationship between essential trace elements and the risk of EEA when considering them as a single element or as mixtures.
摘要：
早期胚胎停滞（EEA）导致接受体外受精（IVF）的不育妇女反复停止新鲜周期。某些必需微量元素[铜（Cu），锌(Zn),妇女体内的硒（Se）和钴（Co）]与EEA认股权证研究的风险有关。
我们的研究旨在调查外周血中Cu水平的相关性，Zn,Se,和Co及其混合物具有EEA的风险。
合肥安徽医科大学第一附属医院生殖中心共有74例EEA（123个IVF周期）和157例对照（180个IVF周期），中国,2017年6月至2020年3月纳入我们的研究.从电子病历中收集人口统计学和临床数据。Cu,Zn,Se,当不育妇女首次进入临床治疗时，使用电感耦合等离子体质谱仪（ICP-MS）测量了取卵当天收集的血液样品中的Co水平。使用广义估计方程（GEE）模型分别评估四种必需微量元素浓度与EEA风险的关联。使用贝叶斯核机回归（BKMR）来探索四种必需微量元素混合物与EEA风险之间的关系。
与对照组相比，病例组不育妇女的硒浓度显着降低。病例组的Co水平明显高于对照组。两组间Cu、Zn浓度差异不显著。基于单金属模型，Co与所有混杂因素调整前后的EEA风险呈正相关（分别为奇数比（OR）=1.72，95％置信区间（CI）：1.18-2.52；OR=2.27，95％CI：1.37-3.77），在校正所有混杂因素之前，硒与EEA风险呈负相关(OR=0.18，95%CI：0.07-0.51)。BKMR分析表明，当所有其他三种金属（Cu，Zn,和Co)被固定在25号，50岁，或第75百分位数，而当所有其他三种金属(Cu，Se和Co)固定在25号，50岁，或第75百分位数。当所有其他金属(Cu，Zn,和Se)固定在25号，50岁，或第75百分位数。此外,发现四种必需微量元素对EEA风险的联合作用有增加的趋势，虽然没有统计学意义。
必需微量元素(Cu，Zn,Se,和Co)可能在一定程度上与EEA的风险相关。当将必需微量元素视为单一元素或混合物时，本研究可能会提供有关必需微量元素与EEA风险之间关系的真实世界观点。
我们的研究旨在调查外周血中Cu水平的相关性，Zn,Se,和Co及其混合物具有EEA的风险。
合肥安徽医科大学第一附属医院生殖中心共有74例EEA（123个IVF周期）和157例对照（180个IVF周期），中国,2017年6月至2020年3月纳入我们的研究.从电子病历中收集人口统计学和临床数据。Cu,Zn,Se,当不育妇女首次进入临床治疗时，使用电感耦合等离子体质谱仪（ICP-MS）测量了取卵当天收集的血液样品中的Co水平。使用广义估计方程（GEE）模型分别评估四种必需微量元素浓度与EEA风险的关联。使用贝叶斯核机回归（BKMR）来探索四种必需微量元素混合物与EEA风险之间的关系。
与对照组相比，病例组不育妇女的硒浓度显着降低。病例组的Co水平明显高于对照组。两组间Cu、Zn浓度差异不显著。基于单金属模型，Co与所有混杂因素调整前后的EEA风险呈正相关（分别为奇数比（OR）=1.72，95％置信区间（CI）：1.18-2.52；OR=2.27，95％CI：1.37-3.77），在校正所有混杂因素之前，硒与EEA风险呈负相关(OR=0.18，95%CI：0.07-0.51)。BKMR分析表明，当所有其他三种金属（Cu，Zn,和Co)被固定在25号，50岁，或第75百分位数，而当所有其他三种金属(Cu，Se和Co)固定在25号，50岁，或第75百分位数。当所有其他金属(Cu，Zn,和Se)固定在25号，50岁，或第75百分位数。此外,发现四种必需微量元素对EEA风险的联合作用有增加的趋势，虽然没有统计学意义。
必需微量元素(Cu，Zn,Se,和Co)可能在一定程度上与EEA的风险相关。当将必需微量元素视为单一元素或混合物时，本研究可能会提供有关必需微量元素与EEA风险之间关系的真实世界观点。
