Abstract：
Spermatozoa acquire fertilization potential during passage through a highly specialized region of the extratesticular ductal system known as the epididymis. In the absence of de novo gene transcription or protein translation, this functional transformation is extrinsically driven via the exchange of varied macromolecular cargo between spermatozoa and the surrounding luminal plasma. Key among these changes is a substantive remodeling of the sperm proteomic architecture, the scale of which has yet to be fully resolved. Here, we have exploited quantitative mass spectrometry-based proteomics to define the extent of changes associated with the maturation of mouse spermatozoa; reporting the identity of >6,000 proteins, encompassing the selective loss and gain of several hundred proteins. Further, we demonstrate epididymal-driven activation of RHOA-mediated signaling pathways is an important component of sperm maturation. These data contribute molecular insights into the complexity of proteomic changes associated with epididymal sperm maturation.
摘要：
精子在通过称为附睾的睾丸外导管系统的高度特化区域时获得受精潜力。在没有从头基因转录或蛋白质翻译的情况下，这种功能转化是通过精子和周围腔内血浆之间不同的大分子货物交换来驱动的。这些变化中的关键是精子蛋白质组结构的实质性重塑，其规模尚未完全解决。这里,我们利用基于质谱的定量蛋白质组学来定义与小鼠精子成熟相关的变化程度;报告>6,000种蛋白质的身份，包括数百种蛋白质的选择性损失和增加。Further,我们证明了附睾驱动的RHOA介导的信号通路激活是精子成熟的重要组成部分.这些数据有助于分子了解与附睾精子成熟相关的蛋白质组变化的复杂性。
