PDF(Pubmed)
Abstract：
Rice bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae (Xoo) is responsible for a significant reduction in rice production. Due to the small impact on the environment, biogenic nanomaterials are regarded as a new type of antibacterial agent. In this research, three colloids of silver nanoparticles (AgNPs) were synthesized with different biological materials such as Arctium lappa fruit, Solanum melongena leaves, and Taraxacum mongolicum leaves, and called Al-AgNPs, Sm-AgNPs and Tm-AgNPs, respectively. The appearance of brown colloids and the UV-Visible spectroscopy analysis proved the successful synthesis of the three colloids of AgNPs. Moreover, FTIR and XRD analysis revealed the formation of AgNPs structure. The SEM and TEM analysis indicated that the average diameters of the three synthesized spherical AgNPs were 20.18 nm, 21.00 nm, and 40.08 nm, respectively. The three botanical AgNPs had the strongest bacteriostatic against Xoo strain C2 at 20 μg/mL with the inhibition zone of 16.5 mm, 14.5 mm, and 12.4 mm, while bacterial numbers in a liquid broth (measured by OD600) decreased by 72.10%, 68.19%, and 65.60%, respectively. Results showed that the three AgNPs could inhibit biofilm formation and swarming motility of Xoo. The ultrastructural observation showed that Al-AgNPs adhered to the surface of bacteria and broke the bacteria. Overall, the three synthetic AgNPs could be used to inhibit the pathogen Xoo of rice bacterial leaf blight.
摘要：
水稻白叶枯病引起的水稻白叶枯病。稻米（Xoo）导致水稻产量显着下降。由于对环境的影响小，生物纳米材料被认为是一种新型的抗菌剂。在这项研究中,用不同的生物材料合成了三种银纳米粒子(AgNPs)胶体,茄子叶，和蒲公英叶，称为Al-AgNPs，Sm-AgNPs和Tm-AgNPs,分别。棕色胶体的出现和紫外可见光谱分析证明了三种AgNP胶体的成功合成。此外，FTIR和XRD分析揭示了AgNPs结构的形成。SEM和TEM分析表明,合成的三种球形AgNPs的平均粒径为20.18nm,21.00nm,和40.08nm，分别。3种植物性AgNPs在20μg/mL时对Xoo菌株C2的抑菌效果最强，抑菌圈为16.5mm，14.5mm,和12.4毫米，而液体肉汤中的细菌数量（通过OD600测量）减少了72.10％，68.19%，和65.60%，分别。结果表明，三种AgNPs均能抑制Xoo的生物膜形成和成群运动。超微结构观察表明，Al-AgNPs粘附在细菌表面并破坏细菌。总的来说,3种合成AgNPs均可用于抑制水稻白叶枯病病原菌Xoo。
