关键词: UPLC-ESI-MS/MS differential metabolites flavonoids kudzu metabolomics

Mesh : Spectrometry, Mass, Electrospray Ionization / methods Pueraria / chemistry Flavonoids / chemistry Isoflavones / analysis Metabolomics / methods Chromatography, High Pressure Liquid / methods Plant Roots / chemistry

来  源:   DOI:10.1002/bmc.5545

Abstract:
Pueraria lobata is a traditional medicinal and edible plant. Its root is often used as a nutritional supplement, but its stems and leaves are often discarded. In this study, the types and contents of compounds in roots, stems and leaves of kudzu were studied by ultra-high-performance liquid chromatography electrospray ionization tandem mass spectrometry. A total of 446 metabolites were identified, which were mainly divided into eight categories; 40 unique compounds were detected in roots. Multivariate statistical analysis showed that there were significant differences in the contents of metabolites in the roots, stems and leaves of kudzu, and the most significant differences were found in the roots and stems. Most flavonoids and terpenoids accumulated in roots, while alkaloids, flavonoid glycosides, lignans and coumarins accumulated in stems and leaves. In addition, isoflavones accumulated least in stems and most in roots. These results improved our understanding of the accumulation of metabolites in kudzu and provided a reference for the full study of the medicinal value of different parts of kudzu.
摘要:
葛属是一种传统的药食植物。它的根通常用作营养补充剂,但是它的茎和叶经常被丢弃。在这项研究中,根中化合物的类型和含量,采用超高效液相色谱-电喷雾串联质谱法对葛根茎叶进行了研究。共鉴定出446种代谢物,主要分为八类;在根中检测到40种独特的化合物。多因素统计分析表明,根中代谢物含量存在显著差异,葛根的茎和叶,在根和茎中发现了最显著的差异。大多数类黄酮和萜类化合物在根中积累,而生物碱,类黄酮苷,木脂素和香豆素在茎和叶中积累。此外,异黄酮在茎中积累最少,在根中积累最多。这些结果提高了我们对葛根代谢产物积累的认识,为全面研究葛根不同部位的药用价值提供了参考。
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