PDF(Pubmed)
Abstract：
The use of functional feeds for farmed fish is now regarded as a key factor in improving fish health and performance against infectious disease. However, the mechanisms by which these nutritional components modulate the immune response are not fully understood. The present study was undertaken to identify the suitability of both primary gut-associated lymphoid tissue (GALT) leucocyte cells and established rainbow trout cell lines as potential alternative methods to test functional feed ingredients prior to full fish feeding trials that can take months to complete. In addition to the primary GALT culture cells, the two rainbow cell lines RTS11 and RTgutGC which are from macrophage and gut epithelial cells, respectively. The cells were stimulated with a variety of pathogen associated molecular patterns (PAMPs) (PHA and Poly I:C) and recombinant rainbow trout IL-1β (rIL-1β), a proinflammatory cytokine, additionally two forms of β-glucan, a prebiotic commonly used aquafeeds were used as stimulants. From this, the suitability of cell models as a health screen for functional feeds was assessed. GALT leucocytes were deemed most effective to act as a health screen over the 4hr time point demonstrating responses to Poly I:C, PHA, and rIL-1β. RTS11 and RTgutGC also responded to the stimulants but did not give a strong T-cell response, most likely reflecting the nature of the cell type as opposed to the mixed cell populations from the primary GALT cell cultures. When stimulated with both forms of β-glucan, GALT leucocytes demonstrated a strong proinflammatory and T-cell response.
摘要：
现在，将功能性饲料用于养殖鱼类被认为是改善鱼类健康和抵抗传染病性能的关键因素。然而,这些营养成分调节免疫反应的机制尚不完全清楚。进行本研究是为了确定原发性肠道相关淋巴组织（GALT）白细胞和已建立的虹鳟鱼细胞系的适用性，作为在可能需要数月才能完成的完整鱼类喂养试验之前测试功能性饲料成分的潜在替代方法。除了原代GALT培养细胞，来自巨噬细胞和肠上皮细胞的两种彩虹细胞系RTS11和RTgutGC，分别。用多种病原体相关分子模式（PAMPs）（PHA和PolyI：C）和重组虹鳟鱼IL-1β（rIL-1β）刺激细胞，一种促炎细胞因子，另外两种形式的β-葡聚糖，一种常用的益生元水生动物被用作兴奋剂。由此,评估了细胞模型作为功能性饲料健康筛查的适用性.GALT白细胞被认为在4小时时间点最有效地充当健康屏幕，证明对PolyI：C的反应，PHA,和rIL-1β。RTS11和RTgutGC也对兴奋剂有反应，但没有产生强烈的T细胞反应，与来自原代GALT细胞培养物的混合细胞群体相反，这很可能反映了细胞类型的性质。当用两种形式的β-葡聚糖刺激时，GALT白细胞表现出强烈的促炎和T细胞反应。
