PDF(Pubmed)
Abstract：
Guvermectin is a recently discovered microbial N9-glucoside cytokinin compound extracted from Streptomyces sanjiangensis NEAU6. Although some research has reported that N9-glucoside cytokinin compounds do not have the activity of cytokinin, it has been noted that guvermectin can promote growth and antifungal activity in Arabidopsis. Maize is an important food crop in the world and exploring the effect of guvermectin on this crop could help its cultivation in regions with adverse environmental conditions such as a high temperature. Here, we investigated the effects of guvermectin seed soaking treatment on the growth of maize at the seedlings stage and its yield attributes with different temperature stresses. The maize (cv. Zhengdan 958) with guvermectin seed soaking treatment were in two systems: paper roll culture and field conditions. Guvermectin seed soaking treated plants had increased plant height, root length, and mesocotyl length at the seedlings stage, and spike weight at maturity in the field. But only root length was increased at the paper roll culture by guvermectin seed soaking treatment. Guvermectin seed soaking treatment reduced the adverse effects on maize seedling when grow at a high temperature. Further experiments showed that, in high temperature conditions, guvermectin treatment promoted the accumulation of heat shock protein (HSP) 17.0, HSP 17.4 and HSP 17.9 in maize roots. Comparative transcriptomic profiling showed there were 33 common differentially expressed genes (DEGs) in guvermectin treated plants under high temperature and room temperature conditions. The DEGs suggested that guvermectin treatment led to the differential modulation of several transcripts mainly related with plant defense, stress response, and terpenoid biosynthesis. Taken together, these results suggested that the guvermectin treatment promoted the growth and tolerance of high temperature stresses, possibly by activation of related pathways. These results show that guvermectin is a novel plant growth regulator and could be developed as an application to maize seeds to promote growth in high temperature environments.
摘要：
Guvermectin是最近发现的一种从三江链霉菌NEAU6中提取的微生物N9-葡萄糖苷细胞分裂素化合物。虽然一些研究报道N9-葡萄糖苷细胞分裂素化合物不具有细胞分裂素的活性，已经注意到，古维菌素可以促进拟南芥的生长和抗真菌活性。玉米是世界上重要的粮食作物，探索古维菌素对该作物的影响可能有助于其在高温等不利环境条件下的种植。这里,研究了不同温度胁迫下，格维菌素浸种处理对玉米苗期生长及其产量特性的影响。玉米(简历。郑单958）与古维菌素种子浸种处理在两个系统中：纸卷培养和田间条件。Guvermectin种子浸种处理的植物有增加的株高，根长,和幼苗阶段的中胚轴长度，和田间成熟时的穗重。但是，通过格维菌素种子浸泡处理，纸卷培养仅增加了根长。古维菌素浸种处理降低了高温条件下对玉米幼苗的不利影响。进一步的实验表明,在高温条件下，古维菌素处理促进了玉米根中热休克蛋白（HSP）17.0，HSP17.4和HSP17.9的积累。比较转录组学分析显示，在高温和室温条件下，格维菌素处理的植物中有33个常见的差异表达基因（DEG）。DEGs表明，古维菌素处理导致几种主要与植物防御有关的转录本的差异调节，应激反应，和萜类生物合成。一起来看,这些结果表明，格维菌素处理促进了生长和对高温胁迫的耐受性，可能是通过激活相关途径。这些结果表明，古维菌素是一种新型的植物生长调节剂，可用于玉米种子在高温环境中促进生长。
