Abstract：
Previous research on antibiotic resistance genes and microorganisms centered on those in urban sewage treatment plants, breeding farms, hospitals and others with serious antibiotic pollution. However, at present, there are evident proofs that antibiotic resistance genes (ARGs) indeed exist in a primitive environment hardly without any human\'s footprints. Accordingly, an original karst mountain swamp ecosystem in Niangniang Mountain, Guizhou, China, including herbaceous swamp, shrub swamp, sphagnum bog and forest swamp, was selected to analyze the physical and chemical parameters of sediments. Moreover, microbial compositions, functions, as well as their connections with ARGs were assayed and analyzed using metagenomic technology. The results showed that there was no significant difference in the dominant microorganisms and ARGs in the four marshes, in which the dominant bacteria phyla were Proteobacteria (37.82 %), Acidobacteriota (22.17 %) and Actinobacteriota (20.64 %); the dominant archaea Euryarchaeota. (1.00 %); and the dominant eukaryotes Ascomycota (0.07 %), with metabolism as their major functions. Based on the ARDB database, the number of ARGs annotated reached 209 including 30 subtypes, and the dominant ARGs were all Bacitracin resistance genes (bacA, 84.77 %). In terms of the diversity of microorganisms and ARGs, the herbaceous swamp ranked the top, and the shrub swamp were at the bottom. Correlation analysis between microorganisms and resistance genes showed that, apart from aac2ic, macB, smeE, tetQ, and tetL, other ARGs were positively correlated with microorganisms. Among them, baca coexisted with microorganisms. Pearson correlation analysis results showed that contrary to ARGs, microorganisms were more affected by environmental factors.
摘要：
以往对抗生素抗性基因和微生物的研究集中在城市污水处理厂，养殖场,医院和其他严重的抗生素污染。然而,目前,有明显的证据表明，抗生素抗性基因（ARGs）确实存在于原始环境中，几乎没有人类的足迹。因此，娘娘山原始的喀斯特山区沼泽生态系统，贵州,中国,包括草本沼泽,灌木沼泽,泥炭沼泽和森林沼泽,选择沉积物的理化参数进行分析。此外，微生物组成，功能,使用宏基因组技术对它们与ARGs的联系进行了分析和分析。结果表明,4个沼泽地的优势微生物和ARGs没有显著差异,其中优势菌门为变形杆菌(37.82%),酸杆菌（22.17％）和放线菌（20.64％）；优势古细菌Euryarchoota。（1.00%）；显性真核生物子囊（0.07%），以新陈代谢为主要功能。基于ARDB数据库，注释的ARG数量达到209个，包括30个亚型，显性ARGs均为杆菌肽抗性基因(bacA，84.77%）。就微生物和ARGs的多样性而言，草本沼泽排名第一，灌木丛沼泽在底部。微生物与抗性基因的相关性分析表明,除了aac2ic，macB,S,tetQ,还有tetL,其他ARGs与微生物呈正相关。其中,baca与微生物共存。皮尔逊相关分析结果表明，与ARGs相反，微生物受环境因素的影响更大。
