Abstract：
With the abuse of antibiotics, bacterial antibiotic resistance is becoming a major public healthcare issue. Natural plants, especially traditional Chinese herbal medicines, which have antibacterial activity, are important sources for discovering potential bacteriostatic agents. This study aimed to develop a fast and reliable method for screening out antimicrobial compounds targeting the MRSA membrane from Psoralea corylifolia Linn. seed. A UPLC-MS/MS method was applied to identify the prenylated flavonoids in major fractions from the extracts of Psoralea corylifolia Linn. seed. The broth microdilution method was used to determine the minimum inhibitory concentrations (MICs) of different fractions and compounds. The morphological and ultrastructural changes of MRSA were determined by scanning electron microscopy (SEM). The membrane-targeting mechanism of the active ingredients was explored by membrane integrity assays, membrane fluidity assays, membrane potential assays, ATP, and ROS determination. We identified eight prenylated flavonoids in Psoralea corylifolia Linn. seed. The antibacterial activity and mechanism studies showed that this type of compound has a unique destructive effect on MRSA cell membranes and does not result in drug resistance. The results revealed that prenylated flavonoids in Psoralea corylifolia Linn. seeds are promising candidates for the development of novel antibiotic agents to combat MRSA-associated infections.
摘要：
随着抗生素的滥用，细菌抗生素耐药性正在成为一个主要的公共卫生问题。天然植物,尤其是中草药，具有抗菌活性，是发现潜在抑菌剂的重要来源。本研究旨在开发一种快速可靠的方法，用于筛选补骨脂中针对MRSA膜的抗菌化合物。种子。采用UPLC-MS/MS方法对补骨脂提取物主要成分中的异戊烯黄酮进行了鉴定。种子。肉汤微量稀释法用于确定不同级分和化合物的最小抑制浓度(MIC)。通过扫描电子显微镜（SEM）确定MRSA的形态和超微结构变化。通过膜完整性分析探讨了活性成分的膜靶向机制，膜流动性测定，膜电位测定，ATP,和ROS测定。我们鉴定了补骨脂中的八种异戊烯化黄酮。种子。抗菌活性和机制研究表明，该类化合物对MRSA细胞膜具有独特的破坏作用，不会产生耐药性。结果表明补骨脂中的异戊烯化黄酮。种子是开发新型抗生素以对抗MRSA相关感染的有希望的候选药物。
