Abstract：
Sperm cryopreservation is of great importance for the poultry industry but still needs to be optimized. The high susceptibility of poultry sperm to cryodamage leads to low fertility rates after cryopreservation. Therefore, the present study aimed at evaluating the effect of including a cryoprotectant, dimethylacetamide (DMA), in the chicken semen freezing extenders at a final concentration of 3%, 6%, or 9% on the post-thawed sperm motility, quality, antioxidant biomarkers, anti-freeze gene expression, and fertilizing ability. Results showed that the total motile sperm, progressivity, and viability were quadratically increased (p < 0.05) in the 6% DMA group. The antioxidant enzyme activity and lipid peroxidation were negatively (p < 0.05) affected by the increase in DMA concentration. Furthermore, some anti-freeze-associated genes such as heat shock protein 70 (HSP70) and ras homolog family member A (RHOA) were linearly and quadratically down-regulated (p < 0.05) with the high concentration of DMA. Finally, the fertility and hatchability rates did not indicate statistical differences between DMA groups. It can be concluded that using the low concentration of 3−6% DMA in the freezing semen extender is preferable to obtain acceptable results in the post-thawed sperm quality and fertility.
摘要：
精子冷冻保存对于家禽业非常重要，但仍需要优化。家禽精子对冷冻损伤的高度敏感性导致冷冻保存后的生育率较低。因此,本研究旨在评估包括冷冻保护剂的效果，二甲基乙酰胺(DMA),在最终浓度为3%的鸡精液冷冻补充剂中，6%,或9%的精子解冻后的活力，质量,抗氧化剂生物标志物，抗冻基因表达，和施肥能力。结果表明，总的活动精子，进步，在6％DMA组中，活力呈二次增加（p＆lt;0.05）。DMA浓度的增加对抗氧化酶活性和脂质过氧化有负面影响（p＆lt;0.05）。此外，一些抗冻相关基因如热休克蛋白70（HSP70）和ras同源蛋白家族成员A（RHOA）在高浓度DMA下呈线性和二次下调（p＆lt;0.05）。最后,生育率和孵化率未显示DMA组之间的统计学差异.可以得出结论，在冷冻精液补充剂中使用低浓度的3-6％DMA是优选的，以在解冻后的精子质量和生育力方面获得可接受的结果。
