PDF(Pubmed)
Abstract：
Antimicrobial compounds, including antibiotics, have been a cornerstone of modern medicine being able to both treat infections and prevent infections in at-risk people, including those who are immune-compromised and those undergoing routine surgical procedures. Their intense use, including in people, animals, and plants, has led to an increase in the incidence of resistant bacteria and fungi, resulting in a desperate need for novel antimicrobial compounds with new mechanisms of action. Many antimicrobial compounds in current use originate from microbial sources, such as penicillin from the fungus Penicillium chrysogenum (renamed by some as P. rubens). Through a collaboration with Aotearoa New Zealand Crown Research Institute Manaaki Whenua-Landcare Research we have access to a collection of thousands of fungal cultures known as the International Collection of Microorganisms from Plants (ICMP). The ICMP contains both known and novel species which have not been extensively tested for their antimicrobial activity. Initial screening of ICMP isolates for activity against Escherichia coli and Staphylococcus aureus directed our interest towards ICMP 477, an isolate of the soil-inhabiting fungus, Aspergillus terreus. In our investigation of the secondary metabolites of A. terreus, through extraction, fractionation, and purification, we isolated nine known natural products. We evaluated the biological activity of selected compounds against various bacteria and fungi and discovered that terrein (1) has potent activity against the important human pathogen Cryptococcus neoformans.
摘要：
抗菌化合物，包括抗生素,已经成为现代医学的基石，能够在高危人群中治疗感染和预防感染，包括那些免疫力低下的人和接受常规外科手术的人。它们的强烈使用，包括人,动物,和植物,导致耐药细菌和真菌的发病率增加，导致迫切需要具有新作用机制的新型抗菌化合物。目前使用的许多抗微生物化合物来源于微生物来源，例如来自真菌产黄青霉的青霉素（被一些人改名为P.rubens）。通过与Aotearoa新西兰皇冠研究所ManaakiWhenua-LandcareResearch的合作，我们可以获得数千种真菌培养物的集合，称为国际植物微生物集（ICMP）。ICMP包含已知的和新的物种,它们的抗微生物活性尚未被广泛测试。初步筛选ICMP分离株对大肠杆菌和金黄色葡萄球菌的活性，使我们对ICMP477感兴趣，ICMP477是土壤中居住的真菌的分离株，土曲霉.在我们对土曲霉次级代谢产物的调查中，通过提取，分馏,和净化，我们分离出9种已知的天然产物.我们评估了所选化合物对各种细菌和真菌的生物活性，并发现土壤酶（1）对重要的人类病原体新生隐球菌具有有效的活性。
