Abstract：
Kinesin-14s constitute a subfamily of the large superfamily of adenosine triphosphate-dependent microtubule-based motor proteins. Kinesin-14s have the motor domain at the C-terminal end of the peptide, playing key roles during spindle assembly and maintenance. Some of them are nonprocessive motors, whereas others can move processively on microtubules. Here, we take budding yeast Cik1-Kar3 and human HSET as examples to study theoretically the dynamics of the processive kinesin-14 motor moving on the single microtubule under load, the dynamics of the motor coupled with an Ndc80 protein moving on the single microtubule, the dynamics of the motor moving in microtubule arrays, and so on. The dynamics of the nonprocessive Drosophila Ncd motor is also discussed. The studies explain well the available experimental data and, moreover, provide predicted results. We show that the processive kinesin-14 motors can move efficiently in microtubule arrays toward the minus ends, and after reaching the minus ends, they can stay there stably, thus performing the function of organizing the microtubules in the bipolar spindle into polar arrays at the spindle poles.
摘要：
Kinesin-14构成了依赖三磷酸腺苷的基于微管的马达蛋白的大型超家族的一个亚家族。Kinesin-14在肽的C末端具有运动结构域，在主轴装配和维护中发挥关键作用。其中一些是非进行性马达，而其他人可以在微管上顺序移动。这里,我们以出芽酵母Cik1-Kar3和人类HSET为例，从理论上研究了持续驱动蛋白14运动在负载下在单个微管上运动的动力学，与Ndc80蛋白在单个微管上运动耦合的运动动力学，电机在微管阵列中运动的动力学，等等。还讨论了非进行性果蝇Ncd运动的动力学。这些研究很好地解释了现有的实验数据，此外,提供预测结果。我们证明，进行性驱动蛋白14电动机可以在微管阵列中有效地向负端移动，到达负端后，他们可以稳定地呆在那里，从而执行将双极主轴中的微管组织成主轴磁极处的极阵的功能。
